Sub-optimal neutralisation of omicron (B.1.1.529) variant by antibodies induced by vaccine alone or SARS-CoV-2 Infection plus vaccine (hybrid immunity) post 6-months

  1. Guruprasad R Medigeshi
  2. Gaurav Batra
  3. Deepika Rathna Murugesan
  4. Ramachandran Thiruvengadam
  5. Souvick Chattopadhyay
  6. Bhabatosh Das
  7. Mudita Gosain
  8. Ayushi
  9. Janmejay Singh
  10. Anantharaj Anbalagan
  11. Heena Shaman
  12. Kamal Pargai
  13. Farha Mehdi
  14. Soon Jyoti Das
  15. Namrata Kahlon
  16. Savita Singh
  17. Pallavi Kshetrapal
  18. Nitya Wadhwa
  19. Anil K Pandey
  20. Shinjini Bhatnagar
  21. Pramod Kumar Garg

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Abstract

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  1. Version published to 10.1016/j.ebiom.2022.103938
  2. ScreenIT

    SciScore for 10.1101/2022.01.04.22268747: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsConsent: Written informed consent was obtained before enrolling the participants.
    IRB: Ethical Approval: The studies were approved by the Institute Ethics Committees of the partnering institutions.
    Sex as a biological variablenot detected.
    RandomizationWe randomly sampled ChAdOx1 nCoV-19 and BBV152 vaccine recipients from the cohort and categorised them in the following four groups: (i) vaccination with two doses of ChAdOx1 nCoV-19 alone, (ii) vaccination with two doses of ChAdOx1 nCoV-19 along with an RT-PCR confirmed natural infection during the delta variant driven surge, (iii) vaccination with two doses of BBV152 alone, (iv) vaccination with two doses of BBV152 along with an RT-PCR confirmed natural infection during the delta variant driven surge.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Anti-SARS-CoV-2 IgG antibody detection: Anti-RBD IgG concentrations in binding antibody units/ml (BAU/ml) in the serum samples were determined by enzyme-linked immunosorbent assay (ELISA) using internal control calibrated against the first WHO international standard for anti-SARS-CoV-2 immunoglobulin (code 20/136).(10) In addition, anti-nucleocapsid antibodies were detected using a qualitative ELISA.
    anti-SARS-CoV-2 immunoglobulin
    suggested: None
    anti-nucleocapsid
    suggested: None
    (13) The FRNT50 of the participants was correlated with quantitative anti-RBD IgG antibody levels (BAU/ml) using Pearson’s correlation coefficient.
    anti-RBD IgG
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Live Virus Focus Reduction Neutralisation Assay: Virus neutralisation assay was performed as described previously with minor changes in the incubation periods.(11) Briefly, plasma samples were serially diluted from 1:20 to 1:640 and virus neutralization was tested in Vero E6 cells.
    Vero E6
    suggested: RRID:CVCL_XD71)
    (12) All virus stocks used in this study were propagated in Calu-3 cells (American Type Culture Collection).
    Calu-3
    suggested: BCRJ Cat# 0264, RRID:CVCL_0609)
    Software and Algorithms
    SentencesResources
    50% neutralisation values were calculated with four-parameter logistic regression using GraphPad Prism (version 9.3.1)
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2022.01.04.22268747v1 on medRxiv