Fusogenicity and neutralization sensitivity of the SARS-CoV-2 Delta sublineage AY.4.2
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SciScore for 10.1101/2022.01.07.475248: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IACUC: This study was approved by the ILE DE FRANCE IV ethical committee.
Consent: At enrolment, written informed consent was collected and participants completed a questionnaire which covered sociodemographic characteristics, virological findings (SARS-CoV-2 RT-PCR results, including date of testing), clinical data (date of symptom onset, type of symptoms, hospitalization), and data related to anti-SARS-CoV-2 vaccination if ever (brand product, date of first and second vaccination).Sex as a biological variable not detected. Randomization The experiments were not randomized and the investigators were not blinded to allocation during experiments and outcome assessment. Blinding The experiments were … SciScore for 10.1101/2022.01.07.475248: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IACUC: This study was approved by the ILE DE FRANCE IV ethical committee.
Consent: At enrolment, written informed consent was collected and participants completed a questionnaire which covered sociodemographic characteristics, virological findings (SARS-CoV-2 RT-PCR results, including date of testing), clinical data (date of symptom onset, type of symptoms, hospitalization), and data related to anti-SARS-CoV-2 vaccination if ever (brand product, date of first and second vaccination).Sex as a biological variable not detected. Randomization The experiments were not randomized and the investigators were not blinded to allocation during experiments and outcome assessment. Blinding The experiments were not randomized and the investigators were not blinded to allocation during experiments and outcome assessment. Power Analysis No statistical methods were used to predetermine sample size. Cell Line Authentication Contamination: Cells were tested negative for mycoplasma. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell-cell fusion assay: For cell–cell fusion assays, 3.5*105 293T cell lines stably expressing GFP1-10 were transfected in suspension with 50 ng of phCMV-SARS-CoV2-spike and 450 ng of pQCXIP-Empty for 30 min at 37°C. 293Tsuggested: NoneVero GFP-11 cells were added at a confluency of 1.5.104 cells per well. Vero GFP-11suggested: NoneTitration of viral stocks was performed on Vero E6, with a limiting dilution technique allowing a calculation of TCID50, or on S-Fuse cells. Vero E6suggested: NoneViruses were sequenced directly on nasal swabs, and after one or two passages on Vero cells. Verosuggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)For studies on transfected cells, HEK293T cells were transfected in suspension using lipofectamine 2000 as per manufacturer’s instruction (ThermoFischer), using 25% of phCMV-SARS-CoV2-spike and 75% of pQCXIP-Empty. HEK293Tsuggested: NoneRecombinant DNA Sentences Resources Plasmids: A codon-optimized version of the reference Wuhan SARS-CoV-2 Spike (GenBank: QHD43416.1) was ordered as a synthetic gene (GeneArt, Thermo Fisher Scientific) and was cloned into a phCMV backbone (GeneBank: AJ318514), by replacing the VSV-G gene. phCMVsuggested: RRID:Addgene_15802)Cell-cell fusion assay: For cell–cell fusion assays, 3.5*105 293T cell lines stably expressing GFP1-10 were transfected in suspension with 50 ng of phCMV-SARS-CoV2-spike and 450 ng of pQCXIP-Empty for 30 min at 37°C. pQCXIP-Emptysuggested: NoneSoftware and Algorithms Sentences Resources Statistical analysis: Flow cytometry data were analyzed with FlowJo v10 software (Becton Dickinson). FlowJosuggested: (FlowJo, RRID:SCR_008520)Figures were drawn on Prism 9 (GraphPad Software). GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Statistical analysis was conducted using GraphPad Prism 9. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT04750720 Recruiting Study of the Kinetics of COVID-19 Antibodies for 24 Months i… Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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