The ChAdOx1 vectored vaccine, AZD2816, induces strong immunogenicity against SARS-CoV-2 beta (B.1.351) and other variants of concern in preclinical studies

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1. Version published to 10.1016/j.ebiom.2022.103902

   Mar 1, 2022
2. 

   ScreenIT

   Jun 12, 2021

   SciScore for 10.1101/2021.06.08.447308: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IACUC: Ethics Statement: Mice were used in accordance with the UK Animals (Scientific Procedures) Act 1986 under project license number P9804B4F1 granted by the UK Home Office with approval from the local Animal Welfare and Ethical Review Board (AWERB) at the University of Oxford.
   Sex as a biological variable	not detected.
   Randomization	Age matched animals were purchased from commercial suppliers as a batch for each experiment and randomly split into groups on arrival at our facility.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Following washing, bound antibodies were detected by addition of a 1 in 5000 …

   SciScore for 10.1101/2021.06.08.447308: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IACUC: Ethics Statement: Mice were used in accordance with the UK Animals (Scientific Procedures) Act 1986 under project license number P9804B4F1 granted by the UK Home Office with approval from the local Animal Welfare and Ethical Review Board (AWERB) at the University of Oxford.
   Sex as a biological variable	not detected.
   Randomization	Age matched animals were purchased from commercial suppliers as a batch for each experiment and randomly split into groups on arrival at our facility.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Following washing, bound antibodies were detected by addition of a 1 in 5000 dilution of alkaline phosphatase (AP)-conjugated goat anti-mouse IgG (Sigma-Aldrich) for 1 hour at RT and addition of p-Nitrophenyl Phosphate, Disodium Salt substrate (Sigma-Aldrich).	anti-mouse IgG suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   R71007), a derivative of HEK293 cells which constitutively express the Tet repressor protein and prevent antigen expression during virus production.	HEK293 suggested: CLS Cat# 300192/p777_HEK293, RRID:CVCL_0045)
   Lentiviral-based SARS-CoV-2 pseudotyped viruses were generated in HEK293T cells incubated at 37 °C, 5% CO2 as previously described 23.	HEK293T suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Animals and Immunizations: Inbred BALB/cOlaHsd (BALB/c) (Envigo) (n=5 to 7 mice per group), were immunized intramuscularly (i.m.) in the musculus tibialis with 108 infectious units (iu) of ChAdOx1 vector.	BALB/c suggested: None
   Recombinant DNA
   Sentences	Resources
   Briefly, cells were seeded at a density of 7.5 × 105 in 6 well dishes, before being transfected with plasmids as follows: 500 ng of SARS-CoV-2 spike (NC_045512, B.1.351, B.1.617.1, B.1.617.2) (Table S1), 600 ng p8.91 (encoding for HIV-1 gag-pol), 600 ng CSFLW	p8.91 suggested: None
   A ‘no glycoprotein’ control was also set up using the pcDNA3.1 vector instead of the SARS-CoV-2 Spike expressing plasmid.	pcDNA3.1 suggested: RRID:Addgene_79663)
   Software and Algorithms
   Sentences	Resources
   Sample acquisition was performed on a Fortessa (BD) and data analyzed in FlowJo V10 (TreeStar).	FlowJo suggested: (FlowJo, RRID:SCR_008520)
   Statistical analysis: All graphs and statistical analysis were performed using Prism v9 (Graphpad).	Prism suggested: (PRISM, RRID:SCR_005375) Graphpad suggested: (GraphPad, RRID:SCR_000306)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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3. 

   Version published to 10.1101/2021.06.08.447308 on bioRxiv

   Jun 9, 2021