Release of infectious virus and cytokines in nasopharyngeal swabs from individuals infected with non-alpha or alpha SARS-CoV-2 variants: an observational retrospective study
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SciScore for 10.1101/2021.05.20.21257393: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: The project was evaluated by the ethics committee “Comité d’éthique de la recherche AP-HP Centre“ affiliated to the AP-HP (Assitance publique des Hopitaux de Paris). Sex as a biological variable not detected. Randomization The experiments were not randomized and the investigators were not blinded to allocation during experiments and outcome assessment. Blinding The experiments were not randomized and the investigators were not blinded to allocation during experiments and outcome assessment. Power Analysis No statistical methods were used to predetermine sample size. Cell Line Authentication Contamination: Cells were tested negative for mycoplasma. Table 2: Resources
Antibodies Sentences Resources Ant… SciScore for 10.1101/2021.05.20.21257393: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: The project was evaluated by the ethics committee “Comité d’éthique de la recherche AP-HP Centre“ affiliated to the AP-HP (Assitance publique des Hopitaux de Paris). Sex as a biological variable not detected. Randomization The experiments were not randomized and the investigators were not blinded to allocation during experiments and outcome assessment. Blinding The experiments were not randomized and the investigators were not blinded to allocation during experiments and outcome assessment. Power Analysis No statistical methods were used to predetermine sample size. Cell Line Authentication Contamination: Cells were tested negative for mycoplasma. Table 2: Resources
Antibodies Sentences Resources Anti-SARS-CoV-2 antibody and Virus stock: The human anti-SARS-CoV2 monoclonal antibody mAb48 recognizes the RBD 20. Anti-SARS-CoV-2suggested: (Thermo Fisher Scientific Cat# PA5-114529, RRID:AB_2890583)anti-SARS-CoV2suggested: NoneExperimental Models: Cell Lines Sentences Resources Briefly, HEK-293T (referred as 293T) cells were acquired from ATCC (ATCC® CRL-3216TM) and tested negative for mycoplasma. HEK-293Tsuggested: None293T Cells stably expressing Spike or control cells were transferred into U-bottom 96-well plates (105 cells/well). 293Tsuggested: NoneSoftware and Algorithms Sentences Resources Statistical analysis: Flow cytometry data were analyzed with FlowJo v10 software (TriStar). FlowJosuggested: (FlowJo, RRID:SCR_008520)Figures were drawn on Prism 9 (GraphPad Software). GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Statistical analysis was conducted using GraphPad Prism 9. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Partial least square regression with “leave-one-out” strategy was performed with R version 4.0.2 on RStudio Desktop 1.3.959 RStudiosuggested: (RStudio, RRID:SCR_000432)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Our study has some limitations. We did not analyse longitudinal samples and the number of samples collected at late time points (after day 10) was scarce. We also did not assess systemic immune responses, since we did not have access to the blood samples of the same individuals. It will be worth determining whether the oral cavity and saliva, important sites for SARS-CoV-2 transmission38, carry different levels of variants. Future work with higher numbers and types of specimen and serial sampling will help further characterizing the virological and immunological determinants of viral transmission. It will be also of great interest to analyze these parameters in asymptomatic and pre-symptomatic individuals, who play a prominent role in viral spreading at the population level.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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