Persistence of immunity to SARS-CoV-2 over time in the ski resort Ischgl

  1. Wegene Borena
  2. Zoltán Bánki
  3. Katie Bates
  4. Hannes Winner
  5. Lydia Riepler
  6. Annika Rössler
  7. Lisa Pipperger
  8. Igor Theurl
  9. Barbara Falkensammer
  10. Hanno Ulmer
  11. Andreas Walser
  12. Daniel Pichler
  13. Matthias Baumgartner
  14. Sebastian Schönherr
  15. Lukas Forer
  16. Ludwig Knabl
  17. Reinhard Würzner
  18. Dorothee von Laer
  19. Jörg Paetzold
  20. Janine Kimpel

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Abstract

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  1. Version published to 10.1016/j.ebiom.2021.103534
  2. ScreenIT

    SciScore for 10.1101/2021.02.19.21252089: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIACUC: Study population, study design and recruitment: The ethical committee (EC) of the Medical University of Innsbruck approved the studies, baseline (Ischgl 1) with EC numbers: 1100/2020 and 1111/2020, which took place between April 21st and 27th, 2020 and follow-up (Ischgl 2) with EC number: 1330/2020, which took place between November 2nd and 8th, 2020.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variableThe covariates used to calculate the Mahalanobis distance were population size, settlement area per square km, share of female population, share of people under 16 years, share of people older than 65 years, number of commuters, share of people with tertiary education, and hotel bed capacity.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    For serostatus d and a individuals were considered as seropositive when they had neutralizing antibodies ≥1:16. Avidity of SARS-CoV-2-specific anti-S IgG: A kit was used according to manufactures instructions to determine of the avidity of anti-S antibodies (Euroimmun AG, Lübeck, Germany, REF: EI 2606-9601 G).
    anti-S IgG
    suggested: None
    anti-S
    suggested: None
    For the detection of remaining antibody, 100 µl of peroxidase-conjugated anti-human IgG were added, incubated for another 30 minutes at 37°C and plates were subsequently washed.
    anti-human IgG
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Shortly, VSVΔG-GFP virus was produced on 293T cells stably expressing a C-terminally truncated version of SARS-CoV-2 spike (Wuhan variant).
    293T
    suggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)
    Four-fold serial dilutions of heat-inactivated plasma were pre-incubated with virus for 1 h at 37°C and subsequently used to infect 293T-ACE2 cells seeded the previous day.
    293T-ACE2
    suggested: RRID:CVCL_YZ65)
    Software and Algorithms
    SentencesResources
    Additionally, each plasma was tested for anti-SARS-CoV-2-N-protein IgG (anti-N IgG) with the Abbott SARS-CoV-2 IgG immunoassay on the ARCHITECT i2000SR system (Abbott, Illinois,
    Abbott
    suggested: (Abbott, RRID:SCR_010477)
    Statistical analysis: Statistical analyses were performed in Stata 16.1 (StataCorp. 2019. Stata Statistical Software:
    StataCorp
    suggested: (Stata, RRID:SCR_012763)
    : StataCorp LLC) and GraphPad Prism version 8.2.0 and 9 (GraphPad Software, Inc., La Jolla, CA, USA).
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.02.19.21252089v1 on medRxiv