Comparing infectivity and virulence of emerging SARS-CoV-2 variants in Syrian hamsters
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SciScore for 10.1101/2021.02.26.433062: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: Infection of hamsters Housing and experimental infections of hamsters have been described15–17 and conducted under supervision of the ethical committee of KU Leuven (license P015-2020). Randomization not detected. Blinding 15–17 In brief, for histological examination, the lungs were fixed overnight in 4% formaldehyde, embedded in paraffin and tissue sections (5 μm) after staining with H&E scored blindly for lung damage (cumulative score of 1 to 3 each for congestion, intra-alveolar hemorrhage, apoptotic bodies in bronchial epithelium, necrotizing bronchiolitis, perivascular edema, bronchopneumonia, perivascular inflammation, peribronchial inflammation, and … SciScore for 10.1101/2021.02.26.433062: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: Infection of hamsters Housing and experimental infections of hamsters have been described15–17 and conducted under supervision of the ethical committee of KU Leuven (license P015-2020). Randomization not detected. Blinding 15–17 In brief, for histological examination, the lungs were fixed overnight in 4% formaldehyde, embedded in paraffin and tissue sections (5 μm) after staining with H&E scored blindly for lung damage (cumulative score of 1 to 3 each for congestion, intra-alveolar hemorrhage, apoptotic bodies in bronchial epithelium, necrotizing bronchiolitis, perivascular edema, bronchopneumonia, perivascular inflammation, peribronchial inflammation, and vasculitis). Power Analysis not detected. Sex as a biological variable In brief, 6 to weeks old female Syrian hamster (Mesocricetus auratus) were sourced from Janvier Laboratories and kept per two in individually ventilated isolator cages. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources All virus stocks were grown on Vero E6 cells and median tissue culture infectious doses (TCID50) defined by titration using the Spearman-Kärber method as decribed. Vero E6suggested: NoneSoftware and Algorithms Sentences Resources All strains B.1-G, B.1.1.7 and B.1.351 isolated in house were subjected to sequencing on a MinION platform (Oxford Nanopore)28 directly from the nasopharyngeal swabs. MinIONsuggested: (MinION, RRID:SCR_017985)Statistical analysis All statistical analyses were performed using GraphPad Prism 9 software (GraphPad, San Diego, CA, USA). GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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