Defective NET clearance contributes to sustained FXII activation in COVID-19-associated pulmonary thrombo-inflammation

  1. Hanna Englert
  2. Chandini Rangaswamy
  3. Carsten Deppermann
  4. Jan-Peter Sperhake
  5. Christoph Krisp
  6. Danny Schreier
  7. Emma Gordon
  8. Sandra Konrath
  9. Munif Haddad
  10. Giordano Pula
  11. Reiner K. Mailer
  12. Hartmut Schlüter
  13. Stefan Kluge
  14. Florian Langer
  15. Klaus Püschel
  16. Kosta Panousis
  17. Evi X. Stavrou
  18. Coen Maas
  19. Thomas Renné
  20. Maike Frye

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Abstract

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  1. Version published to 10.1016/j.ebiom.2021.103382
  2. Version published to 10.1101/2020.12.29.424644v2 on bioRxiv
  3. ScreenIT

    SciScore for 10.1101/2020.12.29.424644: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: Acquisition of patient plasma samples: Heparinized plasma samples were obtained from SARS-CoV-2 positive patients hospitalized at the University Medical Center Hamburg, Germany during the first outbreak in April 2020 (permit number #2322, Ethics Committee of the Hamburg Medical Association, Hamburg, Germany).
    RandomizationThe experiments were not randomized.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    The following antibodies were used: anti-neutrophil elastase (5 μg/ml, ab121595, Abcam)
    anti-neutrophil elastase ( 5
    suggested: None
    Software and Algorithms
    SentencesResources
    The diameter of DNase digestion was measured using Image J.
    Image J
    suggested: (ImageJ, RRID:SCR_003070)
    Statistics: GraphPad Prism was used for graphic representation and statistical analysis of data.
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)
    The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium22 via the PRIDE23 partner repository with the dataset identifier PXD020216.
    ProteomeXchange
    suggested: (ProteomeXchange, RRID:SCR_004055)

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: We found the following clinical trial numbers in your paper:

    IdentifierStatusTitle
    NCT04355364RecruitingEfficacy and Safety of aerosolizedDornase Alfa Administratio…
    NCT04402970CompletedDornase Alfa for ARDS in Patients With SARS-CoV-2
    NCT04445285RecruitingPhase 2 Trial Using rhDNase to Reduce Mortality in COVID-19 …
    NCT04432987RecruitingDornase Alpha for the Treatment of COVID-19
    NCT04359654RecruitingNebulised Dornase Alfa for Treatment of COVID-19
    NCT04402944RecruitingPulmozyme to Improve COVID-19 ARDS Outcomes
    NCT04524962RecruitingStudy of Descartes-30 in Acute Respiratory Distress Syndrome
    NCT04541979RecruitingAerosoliserat DNase for Treatment of Respiratory Failure in …
    NCT04409925RecruitingDISmantling COvid iNduced Neutrophil ExtraCellular Traps (DI…
    NCT04409509CompletedTreatment With CSL312 in Adults With Coronavirus Disease 201…

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  4. Version published to 10.1101/2020.12.29.424644v1 on bioRxiv