Camostat mesylate inhibits SARS-CoV-2 activation by TMPRSS2-related proteases and its metabolite GBPA exerts antiviral activity

Version published to 10.1016/j.ebiom.2021.103255

Mar 1, 2021

SciScore for 10.1101/2020.08.05.237651: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

NIH rigor criteria are not applicable to paper type.

Table 2: Resources

Antibodies
Sentences	Resources
In case of cells transfected with SARS-2-S-encoding vector or empty plasmid, the medium was spiked with anti-VSV-G antibody (supernatant of CRL-2700 cells, 1:1,000) in order to inactivate residual input virus containing VSV-G.	anti-VSV-G suggested: None
Experimental Models: Cell Lines
Sentences	Resources
Transfection of 293T cells was performed by calcium-phosphate precipitation, while Lipofectamine LTX with Plus reagent (Thermo Fisher Scientific) was used for transfection of BHK-21 cells.	293T suggested: None BHK-21 suggested: ATCC Cat# CRL-6282, RRID:CVCL_1914)
For this, HEK-293T cells were first transfected …

SciScore for 10.1101/2020.08.05.237651: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

NIH rigor criteria are not applicable to paper type.

Table 2: Resources

Antibodies
Sentences	Resources
In case of cells transfected with SARS-2-S-encoding vector or empty plasmid, the medium was spiked with anti-VSV-G antibody (supernatant of CRL-2700 cells, 1:1,000) in order to inactivate residual input virus containing VSV-G.	anti-VSV-G suggested: None
Experimental Models: Cell Lines
Sentences	Resources
Transfection of 293T cells was performed by calcium-phosphate precipitation, while Lipofectamine LTX with Plus reagent (Thermo Fisher Scientific) was used for transfection of BHK-21 cells.	293T suggested: None BHK-21 suggested: ATCC Cat# CRL-6282, RRID:CVCL_1914)
For this, HEK-293T cells were first transfected with expression vector for either SARS-2-S or VSV-G (or empty expression vector, control).	HEK-293T suggested: None
Analysis of cell vitality: For the analysis of cell vitality of Calu-3 cells treated with camostat mesylate or FOY-251 the CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) was used.	Calu-3 suggested: None
Infection of Calu-3 cells with authentic SARS-CoV-2: The SARS-CoV-2 isolate hCoV-19/Germany/FI1103201/2020 (GISAID accession EPI-ISL_463008) was isolated at the Institute of Virology, Muenster, Germany, from a patient returning from the Southern Tyrolean ski areas and propagated in Vero-TMPRSS2 cells.	Vero-TMPRSS2 suggested: JCRB Cat# JCRB1818, RRID:CVCL_YQ48)
Software and Algorithms
Sentences	Resources
Statistical analyses: All statistical analyses were performed using GraphPad Prism (version 8.4.2, GraphPad Software, Inc.).	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

Results from OddPub: Thank you for sharing your code and data.

Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

Results from TrialIdentifier: We found the following clinical trial numbers in your paper:

Identifier	Status	Title
NCT04321096	Active, not recruiting	The Impact of Camostat Mesilate on COVID-19 Infection
NCT04353284	Recruiting	Camostat Mesylate in COVID-19 Outpatients
NCT04355052	Recruiting	Open Label Study to Compare Efficacy, Safety and Tolerabilit…
NCT04374019	Recruiting	Novel Agents for Treatment of High-risk COVID-19 Positive Pa…

Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 36. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

Results from rtransparent:

Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
No protocol registration statement was detected.

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Camostat mesylate inhibits SARS-CoV-2 activation by TMPRSS2-related proteases and its metabolite GBPA exerts antiviral activity

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