Persistence of viral RNA, pneumocyte syncytia and thrombosis are hallmarks of advanced COVID-19 pathology

  1. Rossana Bussani
  2. Edoardo Schneider
  3. Lorena Zentilin
  4. Chiara Collesi
  5. Hashim Ali
  6. Luca Braga
  7. Maria Concetta Volpe
  8. Andrea Colliva
  9. Fabrizio Zanconati
  10. Giorgio Berlot
  11. Furio Silvestri
  12. Serena Zacchigna
  13. Mauro Giacca

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Abstract

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  1. Version published to 10.1016/j.ebiom.2020.103104
  2. ScreenIT

    SciScore for 10.1101/2020.06.22.20136358: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: This study was approved by the competent Joint Ethical Committee of the Regione Friuli Venezia
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    After washing with SSC buffer, the presence of SARS-CoV-2 RNA was detected using an anti-DIG alkaline phosphatase (AP) antibody (1:500) (Roche Diagnostics) supplemented with sheep serum (Jackson Immunoresearch) and bovine serum albumin (BSA).
    anti-DIG alkaline phosphatase (AP)
    suggested: None
    Software and Algorithms
    SentencesResources
    , Surfactant B (from Thermo), and against the SARS-CoV-2 Spike protein (from GeneTex).
    Thermo
    suggested: (Thermo Xcalibur, RRID:SCR_014593)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.06.22.20136358v1 on medRxiv