Target capture sequencing of SARS-CoV-2 genomes using the ONETest Coronaviruses Plus

  1. Shing H. Zhan
  2. Sepideh M. Alamouti
  3. Habib Daneshpajouh
  4. Brian S. Kwok
  5. Meng-Hsun Lee
  6. Jaswinder Khattra
  7. Herbert J. Houck
  8. Kenneth H. Rand

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Abstract

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  1. Version published to 10.1016/j.diagmicrobio.2021.115508
  2. ScreenIT

    SciScore for 10.1101/2021.03.25.437083: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: Ethics review: Approval for this study was obtained from the University of Florida Institutional Review Board (IRB202001328).
    RandomizationUsing seqtk v1.3 (https://github/com/lh3/seqtk), we randomly down-sampled (without replacement) the 2×150 nt reads of each ONETest library so that the resulting library had the same number of reads as the matched ARTIC library; each ONETest library was sub-sampled three times in this manner to generate three simulated replicates of the library.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variableAmong the patients, 30 (43%) were male and 40 (57%) were female.

    Table 2: Resources

    Software and Algorithms
    SentencesResources
    Plus RealAmp Kit from OSANG Healthcare Co. Ltd., South Korea), which targets the RdRp, N, and E genes.
    OSANG Healthcare
    suggested: None
    Reads from these libraries were analyzed using a bioinformatics pipeline (v1.3.0; https://github.com/connor-lab/ncov2019-artic-nf) that automates the ARTIC data analysis protocol for Illumina reads (https://artic.network/ncov-2019/ncov2019-bioinformatics-sop.html), which utilizes bwa mem 15, samtools 16, and iVar 17.
    samtools
    suggested: (SAMTOOLS, RRID:SCR_002105)
    Reads were discarded that mapped to the human genome sequence (GRCh38.p13, release 35) using bowtie2 v2.4.2 18.
    bowtie2
    suggested: (Bowtie 2, RRID:SCR_016368)
    The pipeline was implemented in C/C++ and Python using a combination of in-house software and third-party tools, including Biopython v1.78 19, bedtools v2.29.2 20, pybedtools v0.8.1 21, samtools/bcftools/htslib v1.11 16, and Snakemake v5.26.1 22.
    Python
    suggested: (IPython, RRID:SCR_001658)
    Biopython
    suggested: (Biopython, RRID:SCR_007173)
    bedtools
    suggested: (BEDTools, RRID:SCR_006646)
    samtools/bcftools/htslib
    suggested: None
    Snakemake
    suggested: (Snakemake, RRID:SCR_003475)
    Visualization was done in R using ggplot2 23.
    ggplot2
    suggested: (ggplot2, RRID:SCR_014601)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.03.25.437083v1 on bioRxiv