Quantitative, multiplexed, targeted proteomics for ascertaining variant specific SARS-CoV-2 antibody response
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SciScore for 10.1101/2022.02.14.22270845: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Ethics statement: Human sera were obtained from the COVIDsortium Healthcare Workers bioresource [(8–10, 12, 13)] which is approved by the ethical committee of UK National Research Ethics Service (20/SC/0149) and registered on ClinicalTrials.gov (NCT04318314).
Consent: The study conformed to the principles of the Helsinki Declaration, and all subjects gave written informed consent.Sex as a biological variable This included 25 HCW (mean age 44yr, 60% male) with previous laboratory defined evidence of WT SARS-CoV-2 infection and twenty-six HCW (mean age 41y, 54% male) with no laboratory evidence of SARS-CoV-2 infection throughout the initial 16-week longitudinal follow up. Randomization not … SciScore for 10.1101/2022.02.14.22270845: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Ethics statement: Human sera were obtained from the COVIDsortium Healthcare Workers bioresource [(8–10, 12, 13)] which is approved by the ethical committee of UK National Research Ethics Service (20/SC/0149) and registered on ClinicalTrials.gov (NCT04318314).
Consent: The study conformed to the principles of the Helsinki Declaration, and all subjects gave written informed consent.Sex as a biological variable This included 25 HCW (mean age 44yr, 60% male) with previous laboratory defined evidence of WT SARS-CoV-2 infection and twenty-six HCW (mean age 41y, 54% male) with no laboratory evidence of SARS-CoV-2 infection throughout the initial 16-week longitudinal follow up. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antibody ratios >1.1 were considered test positive for the EUROIMMUN SARS-CoV-2 ELISA and >1 was considered test positive for the ROCHE Elecsys anti-SARS-CoV-2 ECLIA following Public Health England evaluation (8–11, 31) The previously reported13,14 cross-sectional, case-controlled vaccine sub-study (n=51) collected samples at a mean/median timepoint of 22d and 20d after administration of the first and second dose of the mRNA vaccine, BNT162b2. anti-SARS-CoV-2suggested: NoneExperimental Models: Cell Lines Sentences Resources VeroE6 cells were seeded in 96-well plates 24h prior to infection. VeroE6suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)Software and Algorithms Sentences Resources Ethics statement: Human sera were obtained from the COVIDsortium Healthcare Workers bioresource [(8–10, 12, 13)] which is approved by the ethical committee of UK National Research Ethics Service (20/SC/0149) and registered on ClinicalTrials.gov (NCT04318314). COVIDsortium Healthcaresuggested: NoneRaw LC-MS/MS data was analysed using Skyline open source software ( Skylinesuggested: (Skyline, RRID:SCR_014080)Exported data were analysed using Microsoft Excel and Graphpad Prism v9. Microsoft Excelsuggested: (Microsoft Excel, RRID:SCR_016137)Graphpad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:One of the limitations with our assay is that we are not able to determine functional complement activation, although precise quantitative detection may be able to funnel further investigation. Our approach using multiplex LC-MS/MS could provide a valuable platform to better enable research in this area. Whilst the LC-MS/MS multiplex assay is a research standard assay, it was designed so it can be easily translated for use in a clinical laboratory setting (7). Simply, by changing the bait to a VoC spike protein, our test can be easily modified to determine antibody mediated immune response for current and emerging VoC. The information obtained will allow us to understand in greater detail an individual’s antibody protection or be used in vaccine design. Furthermore this ‘Bait, Capture and Mass Spec’ approach, can also have applications beyond SAR-CoV-2 for other infectious diseases, or even immune response to novel treatments and autoimmunity.
Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT04318314 Recruiting COVID-19: Healthcare Worker Bioresource: Immune Protection a… Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a protocol registration statement.
Results from scite Reference Check: We found no unreliable references.
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