High-speed large-scale automated isolation of SARS-CoV-2 from clinical samples using miniaturized co-culture coupled to high-content screening
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Abstract
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SciScore for 10.1101/2020.05.14.097295: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The study was approved by the local ethics committee of IHU (Institut Hospitalo-Universitaire) - Méditerranée Infection (No. 2020-01). Randomization Large scale co-culture of clinical samples: We applied this strategy for the detection of SARS-CoV-2 in randomly chosen 104 anonymized nasopharyngeal swab samples. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources 50 µl were then inoculated on a monolayer of Vero E6 cells cultured in 96-well microplates. Vero E6suggested: NoneSoftware and Algorithms Sentences Resources Statistic… SciScore for 10.1101/2020.05.14.097295: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The study was approved by the local ethics committee of IHU (Institut Hospitalo-Universitaire) - Méditerranée Infection (No. 2020-01). Randomization Large scale co-culture of clinical samples: We applied this strategy for the detection of SARS-CoV-2 in randomly chosen 104 anonymized nasopharyngeal swab samples. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources 50 µl were then inoculated on a monolayer of Vero E6 cells cultured in 96-well microplates. Vero E6suggested: NoneSoftware and Algorithms Sentences Resources Statistical analysis: The R Studio® and XLSTAT software were used to perform all statistical tests included in this paper. 6. XLSTATsuggested: (XLSTAT, RRID:SCR_016299)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- No conflict of interest statement was detected. If there are no conflicts, we encourage authors to explicit state so.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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