The impact of BNT162b2 mRNA vaccine on adaptive and innate immune responses

Article activity feed

1. Version published to 10.1016/j.clim.2023.109762

   Oct 1, 2023
2. 

   ScreenIT

   May 8, 2021

   SciScore for 10.1101/2021.05.03.21256520: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Experimental model and subject details: Measurement of antibody levels against RBD and Spike protein: To measure the levels of antibodies against RBD and Spike protein, a fluorescent-bead-based multiplex immunoassay (MIA) was developed as previously described by (Fröberg et al., 2021).	MIA suggested: None
   After incubation, we fixed the cells with 10% formaldehyde and stained the cells with polyclonal rabbit anti-SARS-CoV antibody (Sino Biological) and a secondary peroxidase-labeled …

   SciScore for 10.1101/2021.05.03.21256520: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Experimental model and subject details: Measurement of antibody levels against RBD and Spike protein: To measure the levels of antibodies against RBD and Spike protein, a fluorescent-bead-based multiplex immunoassay (MIA) was developed as previously described by (Fröberg et al., 2021).	MIA suggested: None
   After incubation, we fixed the cells with 10% formaldehyde and stained the cells with polyclonal rabbit anti-SARS-CoV antibody (Sino Biological) and a secondary peroxidase-labeled goat anti-rabbit IgG (Dako).	anti-SARS-CoV suggested: None anti-rabbit IgG suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   After 1 hour incubation, we transferred the mixtures on to Vero-E6 cells and incubated for 8 hours.	Vero-E6 suggested: None
   Software and Algorithms
   Sentences	Resources
   Mean Fluorescent Intensities (MFI) were converted to arbitrary units (AU/ml) by interpolation from a log-5PL-parameter logistic standard curve and log-log axis transformation, using Bioplex Manager 6.2 (Bio-Rad Laboratories) software.	Bioplex suggested: (BioPlex, RRID:SCR_016144) Bio-Rad Laboratories suggested: (Bio-Rad Laboratories, RRID:SCR_008426)
   Statistical analysis: Graphpad Prism 8 was used for all statistical analyses.	Graphpad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

   ---

   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

   ---

   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

   ---

   Results from TrialIdentifier: No clinical trial numbers were referenced.

   ---

   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

   ---

   Results from JetFighter: We did not find any issues relating to colormaps.

   ---

   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

   ---

   Results from scite Reference Check: We found no unreliable references.

   ---

   Read the original source
3. 

   Version published to 10.1101/2021.05.03.21256520 on medRxiv

   May 6, 2021