Strong humoral immune responses against SARS-CoV-2 Spike after BNT162b2 mRNA vaccination with a 16-week interval between doses

  1. Alexandra Tauzin
  2. Shang Yu Gong
  3. Guillaume Beaudoin-Bussières
  4. Dani Vézina
  5. Romain Gasser
  6. Lauriane Nault
  7. Lorie Marchitto
  8. Mehdi Benlarbi
  9. Debashree Chatterjee
  10. Manon Nayrac
  11. Annemarie Laumaea
  12. Jérémie Prévost
  13. Marianne Boutin
  14. Gérémy Sannier
  15. Alexandre Nicolas
  16. Catherine Bourassa
  17. Gabrielle Gendron-Lepage
  18. Halima Medjahed
  19. Guillaume Goyette
  20. Yuxia Bo
  21. Josée Perreault
  22. Laurie Gokool
  23. Chantal Morrisseau
  24. Pascale Arlotto
  25. Renée Bazin
  26. Mathieu Dubé
  27. Gaston De Serres
  28. Nicholas Brousseau
  29. Jonathan Richard
  30. Roberta Rovito
  31. Marceline Côté
  32. Cécile Tremblay
  33. Giulia C. Marchetti
  34. Ralf Duerr
  35. Valérie Martel-Laferrière
  36. Daniel E. Kaufmann
  37. Andrés Finzi

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Abstract

No abstract available

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  1. Version published to 10.1016/j.chom.2021.12.004
  2. Version published to 10.1101/2021.09.17.21263532v2 on medRxiv
  3. ScreenIT

    SciScore for 10.1101/2021.09.17.21263532: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsConsent: EXPERIMENTAL MODEL AND SUBJECT DETAILS: Ethics Statement: All work was conducted in accordance with the Declaration of Helsinki in terms of informed consent and approval by an appropriate institutional board.
    IRB: The study was approved by the respective Institutional Review Boards (no. 19.381 at CHUM and no. 2020/ST/049 at ASST Santi Paolo et Carlo)
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    For evaluation of anti-SARS-CoV-2 antibody-dependent cellular cytotoxicity (ADCC), parental CEM.
    anti-SARS-CoV-2
    suggested: None
    The detection of SARS-CoV-2-antigen specific B cells was done by adding the RBD probes to the antibody cocktail (IgM BUV737, CD24 BUV805, IgG BV421, CD3 BV480, CD56 BV480, CD14 BV480, CD16 BV480, CD20 BV711, CD21 BV786
    CD24
    suggested: (BD Biosciences Cat# 742010, RRID:AB_2871308)
    CD3
    suggested: None
    CD56
    suggested: (BD Biosciences Cat# 566162, RRID:AB_2739559)
    CD14
    suggested: (BD Biosciences Cat# 566141, RRID:AB_2739539)
    CD16
    suggested: (BioLegend Cat# 348805, RRID:AB_2889063)
    CD20
    suggested: (BD Biosciences Cat# 743611, RRID:AB_2741622)
    CD21
    suggested: (BD Biosciences Cat# 742764, RRID:AB_2741029)
    Experimental Models: Cell Lines
    SentencesResources
    Cell lines: 293T human embryonic kidney and HOS cells (obtained from ATCC) were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 5% fetal bovine serum (FBS) (VWR) and 100 μg/ml of penicillin-streptomycin (Wisent).
    HOS
    suggested: None
    293T-ACE2 cell line was previously reported (Prévost et al., 2020).
    293T-ACE2
    suggested: None
    Cell surface staining and flow cytometry analysis: 293T cells were co-transfected with a GFP expressor (pIRES2-GFP, Clontech) in combination with plasmids encoding the full-length Spikes of SARS-CoV-2 variants or Spikes from different Betacoronaviruses.
    293T
    suggested: None
    Recombinant DNA
    SentencesResources
    Cell surface staining and flow cytometry analysis: 293T cells were co-transfected with a GFP expressor (pIRES2-GFP, Clontech) in combination with plasmids encoding the full-length Spikes of SARS-CoV-2 variants or Spikes from different Betacoronaviruses.
    pIRES2-GFP
    suggested: None
    Virus neutralization assay: To produce the pseudoviruses, 293T cells were transfected with the lentiviral vector pNL4.3 R-E- Luc (NIH AIDS Reagent Program) and a plasmid encoding for the indicated S glycoprotein (D614G, B.1.1.7, P.1, B.1.351, B.1.617.2, B.1.526 and SARS-CoV) at a ratio of 10:1.
    pNL4.3
    suggested: None
    Software and Algorithms
    SentencesResources
    All samples were acquired on an LSRII cytometer (BD Biosciences) and data analysis was performed using FlowJo v10.7.1 (Tree Star).
    FlowJo
    suggested: (FlowJo, RRID:SCR_008520)
    Statistics were analyzed using GraphPad Prism version 8.0.1 (GraphPad, San Diego, CA).
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)
    Software scripts and visualization: Edge bundling graphs were generated in undirected mode in R and RStudio using ggraph, igraph, tidyverse,and RColorBrewer packages (R; R studio).
    RStudio
    suggested: (RStudio, RRID:SCR_000432)
    RColorBrewer
    suggested: (RColorBrewer, RRID:SCR_016697)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a protocol registration statement.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  4. Version published to 10.1101/2021.09.17.21263532v1 on medRxiv