Lower Respiratory Tract Myeloid Cells Harbor SARS-Cov-2 and Display an Inflammatory Phenotype
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SciScore for 10.1101/2020.08.11.20171967: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Upon the informed consent of the patient’s legally authorized representative, patients were enrolled in the University of Pittsburgh Acute Lung Injury Registry and Biospecimen Repository (IRB# PRO10110387), which has been described elsewhere.10,11 Longitudinal samples were collected on days 5 and 10 after enrollment. Randomization not detected. Blinding Cell differentials were performed by manual counts of 200 nucleated cells by three separate authors who were blinded to the others’ results – the mean result of the counts was utilized. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Colloidal… SciScore for 10.1101/2020.08.11.20171967: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Upon the informed consent of the patient’s legally authorized representative, patients were enrolled in the University of Pittsburgh Acute Lung Injury Registry and Biospecimen Repository (IRB# PRO10110387), which has been described elsewhere.10,11 Longitudinal samples were collected on days 5 and 10 after enrollment. Randomization not detected. Blinding Cell differentials were performed by manual counts of 200 nucleated cells by three separate authors who were blinded to the others’ results – the mean result of the counts was utilized. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Colloidal gold conjugated secondary antibodies used were goat anti-mouse 18 nm and goat anti-rabbit 6 nm (Jackson ImmunoResearch). anti-mousesuggested: Noneanti-rabbitsuggested: NoneSoftware and Algorithms Sentences Resources Tomographic tilt-series and large-area montaged over-views were acquired using the SerialEM software package (Mastronarde, 2005). SerialEMsuggested: (SerialEM, RRID:SCR_017293)Tomographic data were calculated, analyzed and modeled using the IMOD software package (Kremer et al., 1996; Mastronarde, 2008) on MacPro and iMac Pro computers (Apple, Inc, Cupertino, CA) IMODsuggested: (IMOD, RRID:SCR_003297)Imaris (Bitplane) was used to generate a surface rendered image that illustrated the areas of co-localization in this dataset. Imarissuggested: (Imaris, RRID:SCR_007370)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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