A boost with SARS-CoV-2 BNT162b2 mRNA vaccine elicits strong humoral responses independently of the interval between the first two doses

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1. Version published to 10.1016/j.celrep.2022.111554

   Oct 1, 2022
2. 

   ScreenIT

   Apr 21, 2022

   SciScore for 10.1101/2022.04.18.22273967: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   For evaluation of anti-SARS-CoV-2 antibody-dependent cellular cytotoxicity (ADCC), parental CEM.	anti-SARS-CoV-2 suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Cell surface staining and flow cytometry analysis: 293T cells were co-transfected with a GFP expressor (pIRES2-GFP, Clontech) in combination with plasmids encoding the full-length S of SARS-CoV-2 variants (D614G, Delta and Omicron, BA.1.1 and BA.2), the S2 subunit or the HCoV-HKU1 S.	293T suggested: None
   …

   SciScore for 10.1101/2022.04.18.22273967: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   For evaluation of anti-SARS-CoV-2 antibody-dependent cellular cytotoxicity (ADCC), parental CEM.	anti-SARS-CoV-2 suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Cell surface staining and flow cytometry analysis: 293T cells were co-transfected with a GFP expressor (pIRES2-GFP, Clontech) in combination with plasmids encoding the full-length S of SARS-CoV-2 variants (D614G, Delta and Omicron, BA.1.1 and BA.2), the S2 subunit or the HCoV-HKU1 S.	293T suggested: None
   Recombinant DNA
   Sentences	Resources
   The plasmids encoding the BA.1.1 and BA.2 S were generated by overlapping PCR for mutagenesis of a codon-optimized wild-type SARS-CoV-2 S gene (GeneArt, ThermoFisher) that was synthesized (Biobasic) and cloned in pCAGGS as a template.	pCAGGS suggested: RRID:Addgene_127347)
   Cell surface staining and flow cytometry analysis: 293T cells were co-transfected with a GFP expressor (pIRES2-GFP, Clontech) in combination with plasmids encoding the full-length S of SARS-CoV-2 variants (D614G, Delta and Omicron, BA.1.1 and BA.2), the S2 subunit or the HCoV-HKU1 S.	pIRES2-GFP suggested: None
   Virus neutralization assay: To produce the pseudoviruses, 293T cells were transfected with the lentiviral vector pNL4.3 R-E-Luc (NIH AIDS Reagent Program) and a plasmid encoding for the indicated S glycoprotein (D614G, Delta and Omicron, BA.1.1 and BA.2) at a ratio of 10:1.	pNL4.3 suggested: None
   Software and Algorithms
   Sentences	Resources
   All samples were acquired on an LSRII cytometer (BD Biosciences) and data analysis was performed using FlowJo v10.7.1 (Tree Star).	FlowJo suggested: (FlowJo, RRID:SCR_008520)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Read the original source
3. 

   Version published to 10.1101/2022.04.18.22273967v1 on medRxiv

   Apr 18, 2022