SARS-CoV-2 infections elicit higher levels of original antigenic sin antibodies compared with SARS-CoV-2 mRNA vaccinations
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SciScore for 10.1101/2021.09.30.21264363: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources ELISA plates were washed 3 times with 1x PBS supplemented with 2% Tween (PBS-T) before the addition of goat anti-human IgG conjugated to horseradish peroxidase secondary antibody at a 1:5000 dilution (Jackson ImmunoResearch Laboratories, West Grove, PA: cat. 109-036-098). anti-human IgGsuggested: NoneSerum antibody titers were obtained from a standard curve of either serially diluted monoclonal antibody (CR3022 for SARS-CoV-2 or CR9114 for influenza virus HA starting at 0.5μg/mL) or … SciScore for 10.1101/2021.09.30.21264363: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources ELISA plates were washed 3 times with 1x PBS supplemented with 2% Tween (PBS-T) before the addition of goat anti-human IgG conjugated to horseradish peroxidase secondary antibody at a 1:5000 dilution (Jackson ImmunoResearch Laboratories, West Grove, PA: cat. 109-036-098). anti-human IgGsuggested: NoneSerum antibody titers were obtained from a standard curve of either serially diluted monoclonal antibody (CR3022 for SARS-CoV-2 or CR9114 for influenza virus HA starting at 0.5μg/mL) or serially diluted pooled serum (for OC43 ELISAs). SARS-CoV-2suggested: NoneExperimental Models: Cell Lines Sentences Resources 293F cells were transfected with S1 or S2 encoding plasmids and proteins were purified from cell culture supernatant 6 days later with Ni-NTA resin (Qiagen, Hilden, Germany). 293Fsuggested: RRID:CVCL_D615)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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