SARS-CoV-2 mRNA vaccine induces robust specific and cross-reactive IgG and unequal neutralizing antibodies in naive and previously infected people
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SciScore for 10.1101/2021.06.19.449100: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: All ED HCP personnel who chose to participate in this study provided written informed consent. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The plate was washed three times using wash buffer (TBS containing 0.2% Tween 20), then 50 μL of horseradish peroxidase-conjugated secondary Goat Anti-Human secondary antibody at 1:40,000 dilution in 3% milk was added for 40 minutes at 37°C. Anti-Humansuggested: NoneFor measuring isotype specific antibody, only the respective goat antihuman HRP conjugated IgG, IgM, or IgA was used. antihuman HRP …SciScore for 10.1101/2021.06.19.449100: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: All ED HCP personnel who chose to participate in this study provided written informed consent. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The plate was washed three times using wash buffer (TBS containing 0.2% Tween 20), then 50 μL of horseradish peroxidase-conjugated secondary Goat Anti-Human secondary antibody at 1:40,000 dilution in 3% milk was added for 40 minutes at 37°C. Anti-Humansuggested: NoneFor measuring isotype specific antibody, only the respective goat antihuman HRP conjugated IgG, IgM, or IgA was used. antihuman HRP conjugated IgGsuggested: NoneFor measuring isotype specific antibody, only the respective goat anti-human IgG, IgM, or IgA was used (Cat #109-035-008, 109-035-043, 109-035-011, Jackson ImmunoResearch). only the respective goat anti-human IgGsuggested: Noneanti-human IgGsuggested: (Jackson ImmunoResearch Labs Cat# 109-035-008, RRID:AB_2337579)Meso-scale multiplex surrogate neutralization assay: A multiplexed Meso Scale Discovery (MSD) immunoassay (MSD, Rockville, MD) was used to measure the ACE-2 blocking antibodies to SARS-CoV-2 reference strain and circulating variants (B.1.1.7, P1, B.1.351) using the MSD V-PLEX SARS-CoV-2 Panel 7 (ACE2) kit according to the manufacturer’s instructions. ACE2suggested: NoneTo describe the magnitude and spread of full spike and RBD binding IgG, IgM, and IgA titers, we generated jittered dot plots stratified by antibody isotype, time of sampling (post dose 1 and post dose 2), and prior exposure to SARS-CoV-2. SARS-CoV-2suggested: NoneCrossreactivity between WIV04 and three SARS-CoV-2 variants (B.1.1.7, B.1.351, and P.1), and crossreactivity between SARS-CoV-2 and other endemic human coronaviruses was compared using stratified description of antibody titers and kinetics following each vaccine dose. B.1.351suggested: NoneExperimental Models: Cell Lines Sentences Resources The halo-tagged SARS-CoV-2 NTD antigen (16–305 amino acids, Accession: P0DTC2.1) was designed and expressed in mammalian Expi293 cells as described for RBD antigens. Expi293suggested: RRID:CVCL_D615)Following incubation, serum-virus complexes were added to the plated Vero E6 cells and incubated for 48 hours at 37C with 5% CO2. Vero E6suggested: NoneRecombinant DNA Sentences Resources The bacterial expression construct for full-length SARS-CoV-2 nucleocapsid was a gift from Nicolas Fawzi (Addgene plasmid # 157867; http://n2t.net/addgene:157867; RRID:Addgene_157867)69. detected: RRID:Addgene_157867)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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