High-titer neutralization of Mu and C.1.2 SARS-CoV-2 variants by vaccine-elicited antibodies of previously infected individuals

  1. Takuya Tada
  2. Hao Zhou
  3. Belinda M. Dcosta
  4. Marie I. Samanovic
  5. Amber Cornelius
  6. Ramin S. Herati
  7. Mark J. Mulligan
  8. Nathaniel R. Landau

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Abstract

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  1. Version published to 10.1016/j.celrep.2021.110237
  2. ScreenIT

    SciScore for 10.1101/2021.10.19.463727: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsConsent: The clinical study was conducted at the NYU Vaccine Center with participant’s written consent under IRB-approved protocols (18-02035 and 18-02037).
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Immunoblot analysis: Proteins were analyzed on immunoblots probed with mouse anti-spike monoclonal antibody (1A9) (GeneTex)
    anti-spike
    suggested: (Imported from the IEDB Cat# 1A9, RRID:AB_2848025)
    , anti-p24 monoclonal antibody (AG3.0) and anti-GAPDH monoclonal antibody (Life Technologies) followed by goat anti-mouse HRP-conjugated secondary antibody (Sigma).
    anti-p24
    suggested: None
    anti-p24 monoclonal antibody (AG3.0)
    suggested: None
    anti-GAPDH
    suggested: None
    anti-GAPDH monoclonal antibody (Life Technologies) followed by goat anti-mouse HRP-conjugated secondary antibody (Sigma)
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Binding assay: 293T cells were transfected with mutated spike variant expression vectors using lipofectamine 2000 and seeded in a 96-well plate at 1 × 104 / well.
    293T
    suggested: None
    Neutralization assay by soluble ACE2: Briefly, pseudotyped virus was incubated with serially diluted recombinant soluble ACE2 protein for 1 hour at room temperature and subsequently added to 1 × 104 ACE2.293T cells.
    ACE2.293T
    suggested: None
    Recombinant DNA
    SentencesResources
    The luminescence signal was read in an Envision 2103 microplate luminometer. α-complementation assay: 293T (4 × 106) were cotransfected with pcΔ19S and 5 μg pSCTZ-alpha N85 or variable amounts of pLenti.ACE2-HA and 5 μg of pSCTZ-omega by lipofection with lipofectamine 2000 (Invitrogen).
    pLenti.ACE2-HA
    suggested: None
    pSCTZ-omega
    suggested: None
    Software and Algorithms
    SentencesResources
    Statistical Analysis: All experiments were performed in technical duplicates or triplicates and the data were analyzed using GraphPad Prism 8.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.10.19.463727 on bioRxiv