Inhibitors of VPS34 and fatty-acid metabolism suppress SARS-CoV-2 replication

Article activity feed

1. Version published to 10.1016/j.celrep.2021.109479

   Aug 1, 2021
2. 

   ScreenIT

   Jul 21, 2020

   SciScore for 10.1101/2020.07.18.210211: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Virus and cell lines: Vero E6 (ATCC# CRL-1586), Calu-3 (ATCC# HTB-55), and Caco-2 (ATCC# HTB-37) were maintained in DMEM (Corning) supplemented with 10% heat inactivated fetal bovine serum (FBS; GIBCO).	Caco-2 suggested: None
   For plaque assays, Vero E6 cells were seeded onto a 24-well plate 24 hours before infection.	Vero E6 suggested: None
   Cell viability assay: VeroE6 or Calu-3 cells were seeded in 96-well black walled microplates and …

   SciScore for 10.1101/2020.07.18.210211: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Virus and cell lines: Vero E6 (ATCC# CRL-1586), Calu-3 (ATCC# HTB-55), and Caco-2 (ATCC# HTB-37) were maintained in DMEM (Corning) supplemented with 10% heat inactivated fetal bovine serum (FBS; GIBCO).	Caco-2 suggested: None
   For plaque assays, Vero E6 cells were seeded onto a 24-well plate 24 hours before infection.	Vero E6 suggested: None
   Cell viability assay: VeroE6 or Calu-3 cells were seeded in 96-well black walled microplates and incubated overnight.	Calu-3 suggested: None
   Software and Algorithms
   Sentences	Resources
   Images were rendered in ZenBlue or Imaris Viewer 9.0.	Imaris suggested: (Imaris, RRID:SCR_007370)
   Median time to death calculations were performed by fitting the Boltzmann sigmoid equation to raw kinetic resistance data in Graphpad Prism.	Graphpad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2020.07.18.210211v1 on bioRxiv

   Jul 20, 2020