Prevention and therapy of SARS-CoV-2 and the B.1.351 variant in mice

  1. David R. Martinez
  2. Alexandra Schäfer
  3. Sarah R. Leist
  4. Dapeng Li
  5. Kendra Gully
  6. Boyd Yount
  7. Joy Y. Feng
  8. Elaine Bunyan
  9. Danielle P. Porter
  10. Tomas Cihlar
  11. Stephanie A. Montgomery
  12. Barton F. Haynes
  13. Ralph S. Baric
  14. Michel C. Nussenzweig
  15. Timothy P. Sheahan

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Abstract

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  1. Version published to 10.1016/j.celrep.2021.109450
  2. Version published to 10.1101/2021.01.27.428478v2 on bioRxiv
  3. ScreenIT

    SciScore for 10.1101/2021.01.27.428478: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIACUC: Biocontainment and biosafety: Studies were approved by the UNC Institutional Biosafety Committee approved by animal and experimental protocols in the Baric laboratory.
    RandomizationIn a blinded manner, three random fields of lung tissue were chosen and scored for the following: (A) neutrophils in the alveolar space (none = 0, 1–5 cells = 1, > 5 cells = 2), (B) neutrophils in the interstitial septae (none = 0, 1–5 cells = 1, > 5 cells = 2), (C) hyaline membranes (none = 0, one membrane = 1, > 1 membrane = 2), (D) Proteinaceous debris in air spaces (none = 0, one instance = 1, > 1 instance = 2), (E) alveolar septal thickening (< 2x mock thickness = 0, 2–4x mock thickness = 1, > 4x mock thickness = 2).
    BlindingAnalyses and scoring were performed by a Board Certified Veterinary Pathologist who was blinded to the treatment groups as described previously (Sheahan et al., 2020a).
    Power Analysisnot detected.
    Sex as a biological variableStudy design and treatment groups: For the RDV experiment, a total of N=40, ~20-week-old male and female mice were divided into four groups each with N=10 mice with equal numbers of females and males in each group.

    Table 2: Resources

    Antibodies
    SentencesResources
    Groups of N=20 female mice (N=5 mice treated with C144 + C135, N=5 mice treated with C144, N=5 mice treated with C135, and N=5 mice treated with 3BNC117 + 10-1074) were administered antibody 12 hours before infection, N=20 female mice (N=5 mice treated with C144 + C135, N=5 mice treated with C144, N=5 mice treated with C135, and N=5 mice treated with 3BNC117 + 10-1074) were administered antibody 12hpi (early therapeutic group), N=20 female mice (N=5 mice treated with C144 + C135, N=5 mice treated with C144, N=5 mice treated with C135, and N=5 mice treated with 3BNC117 + 10-1074) were administered antibody 24hpi (mid-late therapeutic group), and N=20 female mice (N=5 mice treated with C144 + C135, N=5 mice treated with C144, N=5 mice treated with C135, and N=5 mice treated with 3BNC117 + 10-1074) were administered antibody 48hpi (late therapeutic group).
    C135
    suggested: None
    Software and Algorithms
    SentencesResources
    Statistics: All statistical analyses were performed using GraphPad Prism 9.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  4. Version published to 10.1101/2021.01.27.428478v1 on bioRxiv