A single intranasal or intramuscular immunization with chimpanzee adenovirus-vectored SARS-CoV-2 vaccine protects against pneumonia in hamsters
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SciScore for 10.1101/2020.12.02.408823: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Five-week old male hamsters were obtained from Charles River Laboratories and housed at Washington University. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Twenty-four hours after virus inoculation, the cells were fixed with formalin, and infected cells were detected by the addition of 100 μL of 1:1000 diluted anti-S protein monoclonal antibody (1C02, gift from Dr. Ellebedy at Washington University) in permeabilization buffer (1x PBS, 2% FBS, 0.2% saponin (Sigma, Cat #S7900)) for 1 h at 20°C or overnight at 4°C, … SciScore for 10.1101/2020.12.02.408823: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Five-week old male hamsters were obtained from Charles River Laboratories and housed at Washington University. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Twenty-four hours after virus inoculation, the cells were fixed with formalin, and infected cells were detected by the addition of 100 μL of 1:1000 diluted anti-S protein monoclonal antibody (1C02, gift from Dr. Ellebedy at Washington University) in permeabilization buffer (1x PBS, 2% FBS, 0.2% saponin (Sigma, Cat #S7900)) for 1 h at 20°C or overnight at 4°C, followed by an anti-human-IgG-HRP antibody (Sigma, Cat. #A6029) in permeabilization buffer for 1 h at 20°C. anti-S proteinsuggested: Noneanti-human-IgG-HRPsuggested: NoneExperimental Models: Cell Lines Sentences Resources Plaque assays were performed on Vero E6 cells in 24-well plates. Vero E6suggested: NoneThe virus-serum mixtures were added to Vero-E6 cell monolayers in 96-well plates and incubated for 1 h at 37°C. Vero-E6suggested: NoneSoftware and Algorithms Sentences Resources Statistical Analysis: The data was analyzed with GraphPad Prism 9.0 and statistical significance was assigned when P values were < 0.05. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.12.02.408823: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Five-week old male hamsters were obtained from Charles River Laboratories and housed at Washington University. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Twenty-four hours after virus inoculation, the cells were fixed with formalin, and infected cells were detected by the addition of 100 µL of 1:1000 diluted anti-S protein monoclonal antibody (1C02, gift from Dr. Ellebedy at Washington University) in permeabilization buffer (1x PBS, 2% FBS, 0.2% saponin (Sigma, Cat #S7900)) for 1 h at 20°C or overnight at 4°C, … SciScore for 10.1101/2020.12.02.408823: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Five-week old male hamsters were obtained from Charles River Laboratories and housed at Washington University. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Twenty-four hours after virus inoculation, the cells were fixed with formalin, and infected cells were detected by the addition of 100 µL of 1:1000 diluted anti-S protein monoclonal antibody (1C02, gift from Dr. Ellebedy at Washington University) in permeabilization buffer (1x PBS, 2% FBS, 0.2% saponin (Sigma, Cat #S7900)) for 1 h at 20°C or overnight at 4°C, followed by an anti-human-IgG-HRP antibody (Sigma, Cat. # A6029) in permeabilization buffer for 1 h at 20°C. anti-S proteinsuggested: (Sigma-Aldrich Cat# A6029, RRID:AB_258272)anti-human-IgG-HRPsuggested: NoneImmunization with ChAd-SARS-CoV-2-S induced high levels of anti-S and anti-RBD IgG(H+L) and IgG2/IgG3 antibodies 21 days later, whereas low or undetectable levels of S- and RBD-specific antibodies were present in samples from ChAd-control immunized animals (Fig 2A-F and Fig S2). anti-Ssuggested: (Novus Cat# H00000191-AP21, RRID:AB_10647247)anti-RBD IgG(H+Lsuggested: NoneIgG2/IgG3suggested: NoneThe antibody response was significantly higher after IN than IM immunization (5 to 7-fold, P < 0.0001 for anti-S and anti-RBD respectively, Fig 2G-H). anti-RBDsuggested: NoneExperimental Models: Cell Lines Sentences Resources Plaque assays were performed on Vero E6 cells in 24-well plates. Vero E6suggested: NoneThe virus-serum mixtures were added to Vero-E6 cell monolayers in 96-well plates and incubated for 1 h at 37°C. Vero-E6suggested: NoneSoftware and Algorithms Sentences Resources The data was analyzed with GraphPad Prism 9.0 and statistical significance was assigned when P values were < 0.05. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.
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