Antiviral drug screen identifies DNA-damage response inhibitor as potent blocker of SARS-CoV-2 replication
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
Article activity feed
-
-
SciScore for 10.1101/2020.06.24.150326: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Biosafety and IRB Approval: Appropriate institutional review boards (IRB) approvals were obtained at UCLA and Cedars-Sinai Medical Center Randomization not detected. Blinding Three to five images per well were quantified for each condition using Image J’s plugin Cell Counter feature was used to count the positively stained cells by a double blinded approach. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources 48 hpi, the cells were fixed and immunostained with anti-dsRNA antibody (J2 clone; Absolute Antibody Inc, USA) to assess viral genome replication (Figure 1C). a…SciScore for 10.1101/2020.06.24.150326: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Biosafety and IRB Approval: Appropriate institutional review boards (IRB) approvals were obtained at UCLA and Cedars-Sinai Medical Center Randomization not detected. Blinding Three to five images per well were quantified for each condition using Image J’s plugin Cell Counter feature was used to count the positively stained cells by a double blinded approach. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources 48 hpi, the cells were fixed and immunostained with anti-dsRNA antibody (J2 clone; Absolute Antibody Inc, USA) to assess viral genome replication (Figure 1C). anti-dsRNAsuggested: (Millipore Cat# MABE1134, RRID:AB_2819101)Experimental Models: Cell Lines Sentences Resources ACE2 entry receptor overexpressing human embryonic kidney 293T cells (293T-ACE2) were established and cultured in the media described above with the presence of puromycin (1 µg/ml). 293Tsuggested: RRID:CVCL_YZ65)SARS-CoV-2 was passaged once in Vero-E6 cells and viral stocks were aliquoted and stored at -80°C. Vero-E6suggested: NoneThe spent media from each well was removed and 100 µl of prepared inoculum was added onto Vero cells. Verosuggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)Software and Algorithms Sentences Resources Image Analysis/Quantification: Microscope images were obtained using the Leica DM IRB and Zeiss Software Program. Zeiss Software Programsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-
