D614G Mutation Alters SARS-CoV-2 Spike Conformation and Enhances Protease Cleavage at the S1/S2 Junction

  1. Sophie M.-C. Gobeil
  2. Katarzyna Janowska
  3. Shana McDowell
  4. Katayoun Mansouri
  5. Robert Parks
  6. Kartik Manne
  7. Victoria Stalls
  8. Megan F. Kopp
  9. Rory Henderson
  10. Robert J. Edwards
  11. Barton F. Haynes
  12. Priyamvada Acharya

Reviewed by

Read the full article

Listed in

Log in to save this article

Abstract

No abstract available

Article activity feed

  1. Version published to 10.1016/j.celrep.2020.108630
  2. ScreenIT

    SciScore for 10.1101/2020.10.11.335299: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    No key resources detected.

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:
    Limitations of this study: In this paper, we study the effect of the D614G mutation on RBD dynamics and susceptibility to furin cleavage. We find that the D614G mutation results in increased furin cleavage susceptibility, which could be responsible for the increased transmissibility of the SARS-CoV-2 with the D614G mutation. It is important to consider though that these results are obtained in the context of an engineered soluble construct and further studies are needed to understand if these effects translate to the native virion context.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.10.11.335299v1 on bioRxiv