Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2
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SciScore for 10.1101/2020.04.23.057265: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Software and Algorithms Sentences Resources The data was automatically collected using SerialEM software (http://bio3d.colorado.edu/SerialEM/). SerialEMsuggested: (SerialEM, RRID:SCR_017293)Image processing: The image drift and anisotropic magnification was corrected using MotionCor2 (Zheng et al., 2017). MotionCor2suggested: (MotionCor2, RRID:SCR_016499)The model was manually corrected for local fit in COOT (Emsley et al., 2010) and the sequence register was corrected based on alignment. COOTsuggested: (Coot, RRID:SCR_014222)The initial model was refined in real space using PHENIX (Adams et al., 2010) with the secondary structural … SciScore for 10.1101/2020.04.23.057265: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Software and Algorithms Sentences Resources The data was automatically collected using SerialEM software (http://bio3d.colorado.edu/SerialEM/). SerialEMsuggested: (SerialEM, RRID:SCR_017293)Image processing: The image drift and anisotropic magnification was corrected using MotionCor2 (Zheng et al., 2017). MotionCor2suggested: (MotionCor2, RRID:SCR_016499)The model was manually corrected for local fit in COOT (Emsley et al., 2010) and the sequence register was corrected based on alignment. COOTsuggested: (Coot, RRID:SCR_014222)The initial model was refined in real space using PHENIX (Adams et al., 2010) with the secondary structural restraints and Ramachandran restrains applied. PHENIXsuggested: (Phenix, RRID:SCR_014224)The model was further adjusted and refined iteratively for several rounds aided by the stereochemical quality assessment using MolProbity (Chen et al., 2010). MolProbitysuggested: (MolProbity, RRID:SCR_014226)Structural figures were rendered by either CHIMERA (Pettersen et al., 2004) or PyMOL (https://pymol.org/). PyMOLsuggested: (PyMOL, RRID:SCR_000305)Images were taken using a Vilber Fusion system and analyzed with the Image J software. Image Jsuggested: (ImageJ, RRID:SCR_003070)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 30. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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