Cross-reactive Antibody Response between SARS-CoV-2 and SARS-CoV Infections
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SciScore for 10.1101/2020.03.15.993097: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Methods Recruitment of patients and specimen collections Patients with RT-PCR confirmed COVID-19 disease at the Infectious Disease Centre of the Princess Margaret Hospital , Hong Kong , were invited to participate in the study after providing informed consent . Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Whether these non-neutralizing antibody responses will lead to antibody-dependent disease enhancement needs to be addressed in the future. antibody-dependent disease…SciScore for 10.1101/2020.03.15.993097: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Methods Recruitment of patients and specimen collections Patients with RT-PCR confirmed COVID-19 disease at the Infectious Disease Centre of the Princess Margaret Hospital , Hong Kong , were invited to participate in the study after providing informed consent . Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Whether these non-neutralizing antibody responses will lead to antibody-dependent disease enhancement needs to be addressed in the future. antibody-dependent diseasesuggested: NoneCross-reactivity in mouse infection and vaccination To further investigate the cross-reactivity of antibody responses to SARS-CoV-2 and SARS-CoV , we analyzed the antibody response of plasma collected from mice infected or immunized with SARS-CoV-2 or SARS-CoV ( n = 5 or 6 per experimental and control groups) . SARS-CoVsuggested: NoneOn the contrary , non-neutralizing antibody responses can also lead to antibody-dependent enhancement ( ADE ) of infection as reported in other coronaviruses ( Tseng et al. , 2012; Wang et al. , 2014; Weiss and Scott , 1981) . antibody-dependent enhancement ( ADEsuggested: NoneAfter extensive washing with PBS containing 0.1% Tween 20, each well in the plate was further incubated with the HRPsheep anti-mouse or anti-human secondary antibody (1:5000, GE Healthcare) for 1 hour at 37°C. anti-humansuggested: NoneExperimental Models: Cell Lines Sentences Resources Microneutralization assay Plasma samples were diluted in serial two-fold dilutions commencing with a dilution of 1:10, and mixed with equal volumes of SARS-CoV or SARS-CoV-2 at a dose of 200 tissue culture infective doses 50% (TCID50) determined by Vero and Vero E6 cells respectively. Vero E6suggested: CVCL_XD71Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
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