Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy
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SciScore for 10.1101/2020.09.28.20201947: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources SARS-CoV-2 stocks were propagated in Vero CCL-81 cells. Vero CCL-81suggested: NoneHCoV-NL63 virus culture: Isolate Amsterdam I of HCoV-NL63 (NR-470, BEI Resources) was propagated in Huh7.5.1-ACE2 cells. Huh7.5.1-ACE2suggested: NoneHCoV-OC43 virus culture: HCoV-OC43 (VR-1558, ATCC) was propagated in Vero E6 cells. Vero E6suggested: RRID:CVCL_XD71)RNA extraction: RNA was extracted from SARS-CoV-2, HCoV-NL63, HCoV-OC43, Influenza A, and … SciScore for 10.1101/2020.09.28.20201947: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources SARS-CoV-2 stocks were propagated in Vero CCL-81 cells. Vero CCL-81suggested: NoneHCoV-NL63 virus culture: Isolate Amsterdam I of HCoV-NL63 (NR-470, BEI Resources) was propagated in Huh7.5.1-ACE2 cells. Huh7.5.1-ACE2suggested: NoneHCoV-OC43 virus culture: HCoV-OC43 (VR-1558, ATCC) was propagated in Vero E6 cells. Vero E6suggested: RRID:CVCL_XD71)RNA extraction: RNA was extracted from SARS-CoV-2, HCoV-NL63, HCoV-OC43, Influenza A, and Influenza B viral supernatant via RNA STAT-60 (AMSBIO) and the Direct-Zol RNA MiniPrep Kit (Zymo Research). HCoV-NL63suggested: RRID:CVCL_RW88)Experimental Models: Organisms/Strains Sentences Resources HCoV-OC43 virus culture: HCoV-OC43 (VR-1558, ATCC) was propagated in Vero E6 cells. HCoV-OC43 virus culture: HCoV-OC43suggested: NoneSoftware and Algorithms Sentences Resources Mobile phone image acquisition and analysis: During typical device operation, an RGB image was acquired every 30 seconds for the duration of 1 hour and the images were analyzed offline using a custom MATLAB code. MATLABsuggested: (MATLAB, RRID:SCR_001622)Quantification and statistical analysis: Data in Figures 1-3 were processed and visualized using GraphPad Prism. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.09.28.20201947: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources We performed the same assay with full-length SARS-CoV-2 RNA isolated from the supernatant of SARS-CoV-2-infected Vero CCL-81 cells and found the limit of detection was 270 full-length viral copies/µL (Slope 12.4 ± SE 0.3) (Figure Vero CCL-81suggested: NoneResults from OddPub: We did not detect open data. We also …
SciScore for 10.1101/2020.09.28.20201947: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources We performed the same assay with full-length SARS-CoV-2 RNA isolated from the supernatant of SARS-CoV-2-infected Vero CCL-81 cells and found the limit of detection was 270 full-length viral copies/µL (Slope 12.4 ± SE 0.3) (Figure Vero CCL-81suggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.
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