Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19
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SciScore for 10.1101/2020.03.24.004655: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Normal human bronchial epithelial (NHBE) cells (Lonza, CC-2540 Lot# 580580) were isolated from a 79-year-old Caucasian female and were maintained in bronchial epithelial growth media (Lonza, CC-3171) supplemented with BEGM SingleQuots as per the manufacturer’s instructions (Lonza, CC-4175) at 37°C and 5% CO2. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell Culture: Human adenocarcinomic alveolar basal epithelial (A549) cells (ATCC, CCL-185), Madin-Darby Canine Kidney (MDCK) cells (ATCC, … SciScore for 10.1101/2020.03.24.004655: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Normal human bronchial epithelial (NHBE) cells (Lonza, CC-2540 Lot# 580580) were isolated from a 79-year-old Caucasian female and were maintained in bronchial epithelial growth media (Lonza, CC-3171) supplemented with BEGM SingleQuots as per the manufacturer’s instructions (Lonza, CC-4175) at 37°C and 5% CO2. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell Culture: Human adenocarcinomic alveolar basal epithelial (A549) cells (ATCC, CCL-185), Madin-Darby Canine Kidney (MDCK) cells (ATCC, CCL-34), African green monkey kidney epithelical Vero E6 cells (ATCC, CRL-1586) and African green monkey kidney epithelical BS-C-1 cells (ATCC, CCL-26) were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% Fetal Bovine Serum (FBS, Corning). A549suggested: NoneMDCKsuggested: NoneBS-C-1suggested: NoneRecombinant GFP-expressing human respiratory syncytial virus, strain A2 (rgRSV[224]) was generously provided by Dr. M. Peeples (OSU) and was described previously12. rgRSV[224] was grown in BSC-1 cells in in DMEM supplemented with 2% FBS, 4.5 g/L D-glucose and 4 mM L-glutamine. BSC-1suggested: ATCC Cat# CCL-26, RRID:CVCL_0607)SARS-CoV-2 was propagated in Vero E6 cells in DMEM supplemented with 2% FBS, 4.5 g/L D-glucose, 4 mM L-glutamine, 10 mM Non-Essential Amino Acids, 1 mM Sodium Pyruvate and 10 mM HEPES. Vero E6suggested: RRID:CVCL_XD71)Software and Algorithms Sentences Resources Gene set enrichment analysis (GSEA) was performed on gene expression profiles from mock and SARS-CoV-2 treated cells comparing to a composite list of genes involved in Type-I IFN response (GO:0035457, GO:0035458, GO:0035455, GO:0035456, GO:0034340, and HALLMARK_INTERFERON_ALPHA_RESPONSE)16. Gene set enrichment analysissuggested: (Gene Set Enrichment Analysis, RRID:SCR_003199)Alignments to viral genomes was performed using bowtie2. bowtie2suggested: (Bowtie 2, RRID:SCR_016368)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- No conflict of interest statement was detected. If there are no conflicts, we encourage authors to explicit state so.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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