Force-dependent stimulation of RNA unwinding by SARS-CoV-2 nsp13 helicase
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
Article activity feed
-
SciScore for 10.1101/2020.07.31.231274: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Enzyme parameters KM, kcat and Hill coefficients (h) were determined by fitting the plot of initial rates (first ~150 s) against substrate concentration to the Michaelis-Menten equation or the Hill equation using Prism v.6.0 (GraphPad Software, Prismsuggested: (PRISM, RRID:SCR_005375)Data were fit using a sigmoidal dose dependent equation: using Prism v.6.0 (GraphPad Software, Inc)
GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Single-molecule data were analyzed in MATLAB … SciScore for 10.1101/2020.07.31.231274: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Enzyme parameters KM, kcat and Hill coefficients (h) were determined by fitting the plot of initial rates (first ~150 s) against substrate concentration to the Michaelis-Menten equation or the Hill equation using Prism v.6.0 (GraphPad Software, Prismsuggested: (PRISM, RRID:SCR_005375)Data were fit using a sigmoidal dose dependent equation: using Prism v.6.0 (GraphPad Software, Inc)
GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Single-molecule data were analyzed in MATLAB (Natick, MA). MATLABsuggested: (MATLAB, RRID:SCR_001622)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-
-