Harnessing recombinase polymerase amplification for rapid multi-gene detection of SARS-CoV-2 in resource-limited settings

  1. Dounia Cherkaoui
  2. Da Huang
  3. Benjamin S. Miller
  4. Valérian Turbé
  5. Rachel A. McKendry

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Abstract

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  1. Version published to 10.1016/j.bios.2021.113328
  2. ScreenIT

    SciScore for 10.1101/2021.02.17.21251732: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Experimental Models: Cell Lines
    SentencesResources
    The coronavirus specificity panel (SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-NL63, HCoV-OC43 and HCoV-229E) was obtained from the European Virus Archive (
    HCoV-NL63
    suggested: RRID:CVCL_RW88)
    Software and Algorithms
    SentencesResources
    Then, the average values of duplicates were plotted on GraphPad Prism along with error bars, corresponding to the standard deviation.
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)
    The fraction of positive reactions (reactions which reached the threshold in less than 20 minutes) was calculated separately for both genes and probit analysis was done on Matlab (R2020b).
    Matlab
    suggested: (MATLAB, RRID:SCR_001622)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.02.17.21251732v1 on medRxiv