Nanozyme chemiluminescence paper test for rapid and sensitive detection of SARS-CoV-2 antigen
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SciScore for 10.1101/2020.06.05.131748: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Fiberglass pads, absorbent pads and plastic boards were purchased from Shanghai Kinbio Tech. Co. Ltd. Ab1, Ab2, TP52, RP01 antibodies against SARS-CoV-2 spike antigen were provided by Academy of Military Medical Sciences and Sino Biological Inc. (Beijing, China). TP52suggested: NoneAnti-Spike antibodies were generated by sorting single memory B cells as previously reported19. Anti-Spikesuggested: None, anti-CD19, anti-CD38, anti-CD27 and anti-His antibodies. …SciScore for 10.1101/2020.06.05.131748: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Fiberglass pads, absorbent pads and plastic boards were purchased from Shanghai Kinbio Tech. Co. Ltd. Ab1, Ab2, TP52, RP01 antibodies against SARS-CoV-2 spike antigen were provided by Academy of Military Medical Sciences and Sino Biological Inc. (Beijing, China). TP52suggested: NoneAnti-Spike antibodies were generated by sorting single memory B cells as previously reported19. Anti-Spikesuggested: None, anti-CD19, anti-CD38, anti-CD27 and anti-His antibodies. anti-CD19suggested: Noneanti-CD38,suggested: Noneanti-CD27suggested: Noneanti-Hissuggested: NoneFabrication of nanozyme-based chemiluminescence test paper: Anti-S-RBD antibody pairs were screening using ELISA method and nanozyme-based colorimetric strips15. Anti-S-RBDsuggested: NoneThe paired capture antibody of S-RBD (S-cAb) was immobilized on a nitrocellulose membrane at 1 mg/ml to form the test line (T-line), as well as the anti-human IgG antibody as the control line (C-line). anti-human IgGsuggested: NoneNanozyme probes uncombined with antigen bound with the control IgG antibody at C-line. control IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources A cDNA sequence encoding S-RBD was expressed containing both N-terminal natural signal peptide as well as a 6×His tag at the C-terminus, after transfection into HEK293 cells. HEK293suggested: CLS Cat# 300192/p777_HEK293, RRID:CVCL_0045)Rapid testing of pseudo-SARS-CoV-2: The pseudo-SARS-CoV-2 were packaged by transfecting 293T cells with a HIV-1-based retroviral vector coding SARS-CoV-2 spike S1 protein and luciferase reporter gene. 293Tsuggested: NoneSoftware and Algorithms Sentences Resources The antibody binding affinity for S-RBD was evaluated by BIAcore 8K. BIAcoresuggested: (Biacore T100 System, RRID:SCR_019679)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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