Array-based analysis of SARS-CoV-2, other coronaviruses, and influenza antibodies in convalescent COVID-19 patients
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SciScore for 10.1101/2020.06.15.153064: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Sera were drawn under a protocol approved by the University of Rochester Medical Center Institutional Review Board. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication Authentication: During development, several printing concentrations and/or solution pH values of each antigen were tested, along with sugar additives (glycerol, trehalose) in order to optimize spot uniformity and morphology as well as initial probe thickness. Table 2: Resources
Antibodies Sentences Resources Secondary antibodies were diluted to 1 μg/mL for both goat α-hIgG (Jackson Immunoresearch) and rabbit … SciScore for 10.1101/2020.06.15.153064: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Sera were drawn under a protocol approved by the University of Rochester Medical Center Institutional Review Board. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication Authentication: During development, several printing concentrations and/or solution pH values of each antigen were tested, along with sugar additives (glycerol, trehalose) in order to optimize spot uniformity and morphology as well as initial probe thickness. Table 2: Resources
Antibodies Sentences Resources Secondary antibodies were diluted to 1 μg/mL for both goat α-hIgG (Jackson Immunoresearch) and rabbit α-hIgM (Rockland, Inc.) in Adarza diluent. α-hIgGsuggested: Noneα-hIgMsuggested: NoneWhile anti-FITC spots were designed to serve as an intra-chip normalizer, these were not used as such due to the unexpected presence of anti-goat IgG antibodies in some single donor human serum samples. anti-FITCsuggested: Noneanti-goat IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources SARS-CoV2 nucleocapsid expressed in mammalian cells was obtained from RayBiotech, while HCoV-229E and HCoV-OC43 spike proteins (baculovirus-expressed) were obtained from Sino Biological. HCoV-229Esuggested: NoneSoftware and Algorithms Sentences Resources Aminereactive substrates for fabrication of AIR arrays were provided by Adarza BioSystems, Inc. Adarza BioSystemssuggested: NoneThe median intensity values were converted to median thickness values using a best-fit line to an experimentally derived reflectance model.14 Then, the median thickness values were further processed in Microsoft Excel as described below, and are referred to simply as “thickness” hereafter. Microsoft Excelsuggested: (Microsoft Excel, RRID:SCR_016137)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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