From a recombinant key antigen to an accurate, affordable serological test: Lessons learnt from COVID-19 for future pandemics
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SciScore for 10.1101/2020.07.13.20152884: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Sample collection from human subjects: Samples collected at the State Hematology Institute Hemorio followed a protocol approved by the local ethics committee (CEP Hemorio; approval #4008095).
Consent: Samples collected at UFRJ COVID Screening and Diagnostic Center followed a protocol approved by the national ethics committee (CONEP, Brazil; protocol #30161620000005257; approval #3953368): subjects were initially interviewed and, if they accepted to participate, they signed the informed consent, answered a questionnaire (addressing demographic data, onset and type of symptoms, history of travel abroad, among other information) and had blood (venous blood …SciScore for 10.1101/2020.07.13.20152884: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Sample collection from human subjects: Samples collected at the State Hematology Institute Hemorio followed a protocol approved by the local ethics committee (CEP Hemorio; approval #4008095).
Consent: Samples collected at UFRJ COVID Screening and Diagnostic Center followed a protocol approved by the national ethics committee (CONEP, Brazil; protocol #30161620000005257; approval #3953368): subjects were initially interviewed and, if they accepted to participate, they signed the informed consent, answered a questionnaire (addressing demographic data, onset and type of symptoms, history of travel abroad, among other information) and had blood (venous blood and/or finger prick) and nasopharyngeal swab collected.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Presence of S protein in the supernatants was determined by spot blots: 3 µL of each sample was applied to nitrocellulose membranes, serum of SARS-COV-2 convalescent patients (1:1000) was used as primary antibody, followed by incubation with anti-human IgG(Fc) HRP-labeled antibody (Sigma, #SAB3701282) and finally addition of chemiluminescent ECL reagent (BioRad). anti-human IgG(Fcsuggested: NoneFor Western blots, serum of SARS-COV-2 convalescent patients (1:1000) was used as primary antibody, followed by incubation with anti-human IgG (Fc) HRP-labeled antibody (Sigma, #SAB3701282) and then chemiluminescent ECL reagent (BioRad). anti-human IgGsuggested: NoneNext, 50 μL of 1:8000 goat anti-human IgG (Fc) HRP-labeled antibody (Sigma, #SAB3701282) was added to the plate and incubated for 1.5 hours at RT. 1:8000 goat anti-human IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Stable cell line generation: HEK293-3F6 cells (NRC Canada) growing in suspension in the chemically-defined, animal component-free HEK-TF (Xell AG) culture medium were stably transfected by lipofection using a broad-spectrum reagent (Lipofectamine 3000, Thermo Fisher) as described previously4. HEK293-3F6suggested: NoneVirus-serum mixture was inoculated into confluent monolayers of Vero cells seeded in 12-well tissue culture plates. Verosuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.07.13.20152884: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources An ELISA was then established for the detection of anti-S antibodies in serum, plasma and whole blood eluates (S-UFRJ ELISA). anti-Ssuggested: NoneExperimental Models: Cell Lines Sentences Resources We expressed the soluble ectodomain of the spike (S) protein of SARSCOV-2 in a stabilized prefusion conformation [3] in serum-free, suspension-adapted HEK293-3F6 cells. …SciScore for 10.1101/2020.07.13.20152884: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources An ELISA was then established for the detection of anti-S antibodies in serum, plasma and whole blood eluates (S-UFRJ ELISA). anti-Ssuggested: NoneExperimental Models: Cell Lines Sentences Resources We expressed the soluble ectodomain of the spike (S) protein of SARSCOV-2 in a stabilized prefusion conformation [3] in serum-free, suspension-adapted HEK293-3F6 cells. HEK293-3F6suggested: NoneDue to the challenges posed by the pandemics, such as the urgency and the disruption of international supply chains for reagents and synthetic gene constructs, we decreased time and costs by using an old-fashioned technique of co-transfecting the plasmid containing the S gene and an intellectual property-free plasmid higher expression than transiently transfected HEK293 or CHO-K1 cells was generated and banked within 24 days post-transfection, and stability of expression by this cell line has so far been proven for up to 100 days post-transfection (Fig. 1a), thus making it feasible to develop less costly, long-lasting batch-refeed or perfusion technologies for cell culture. CHO-K1suggested: NoneAbout SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.
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