SARS-CoV-2 triggers DNA damage response in Vero E6 cells
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SciScore for 10.1101/2021.09.08.459535: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Primers: Immunoblotting and Antibodies: Protein lysates from SARS-CoV-2 infected Vero E6 cells were harvested in 2x Laemmli sample buffer (Bio-Rad) containing 2-Mercaptoethanol (Fisher Scientific). Antibodiessuggested: NoneMembranes were blotted with the following antibodies: Actin (Invitrogen, MA1744 Actinsuggested: (Thermo Fisher Scientific Cat# MA1-744, RRID:AB_2223496)Membranes were washed three times for five minutes each with PBS (Cytia) with 0.1% Tween-20, before and after … SciScore for 10.1101/2021.09.08.459535: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Primers: Immunoblotting and Antibodies: Protein lysates from SARS-CoV-2 infected Vero E6 cells were harvested in 2x Laemmli sample buffer (Bio-Rad) containing 2-Mercaptoethanol (Fisher Scientific). Antibodiessuggested: NoneMembranes were blotted with the following antibodies: Actin (Invitrogen, MA1744 Actinsuggested: (Thermo Fisher Scientific Cat# MA1-744, RRID:AB_2223496)Membranes were washed three times for five minutes each with PBS (Cytia) with 0.1% Tween-20, before and after incubating with secondary antibody for one hour, with donkey anti-mouse (IRDye 680RD, Li-COR BioSciences) or goat anti-rabbit (IRDye 800CW, Li-COR Biosciences), at 1:20,000 with 0.01% SDS and 0.1% Tween-20. anti-mousesuggested: Noneanti-rabbitsuggested: NoneExperimental Models: Cell Lines Sentences Resources Vero E6 cells were infected with SARS-CoV-2 WA1 at an MOI of 0.01 and incubated for 48 hours before collecting the cells for further downstream processing. Vero E6suggested: NoneSoftware and Algorithms Sentences Resources RT-qPCR and Telomere Length Assay: Isolated RNA was quantified using the Nanodrop-2000 (ThermoFisher), and then diluted using RNase/DNase free water (VWR Life Sciences) until all the concentration of RNA in each sample was 10ng/µL. ThermoFishersuggested: (ThermoFisher; SL 8; Centrifuge, RRID:SCR_020809)The StepOnePlus software was used to analyze that data and GraphPad Prism 9.0.1 was used for statistical analysis and to the plot the results. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Membranes were imaged with the Li-COR Odyssey CLx, and images were analyzed with Image Studio software. Image Studiosuggested: (Image Studio Lite, RRID:SCR_013715)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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