Therapeutic effect of CT-P59 against SARS-CoV-2 South African variant
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SciScore for 10.1101/2021.04.27.441707: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: For in vivo study, NMC-nCoV02 (S clade) and hCoV-19/Korea/KDCA55905/202 (B.1.351) were provided by National Culture Collection for Pathogens. 2.2.
IACUC: All animal cares were performed strictly following the animal care guideline and experiment protocols approved by Institutional Animal Care and Use Committee (IACUC) in Chungbuk National University (Sex as a biological variable Ferret study: Groups of 14- to 16-month-old female ferrets ( Randomization not detected. Blinding Cough, rhinorrhea and movement were scored and analyzed for each animal in blind manner. 2.8. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Fol… SciScore for 10.1101/2021.04.27.441707: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: For in vivo study, NMC-nCoV02 (S clade) and hCoV-19/Korea/KDCA55905/202 (B.1.351) were provided by National Culture Collection for Pathogens. 2.2.
IACUC: All animal cares were performed strictly following the animal care guideline and experiment protocols approved by Institutional Animal Care and Use Committee (IACUC) in Chungbuk National University (Sex as a biological variable Ferret study: Groups of 14- to 16-month-old female ferrets ( Randomization not detected. Blinding Cough, rhinorrhea and movement were scored and analyzed for each animal in blind manner. 2.8. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Following blocking, serially diluted CT-P59 were incubated, followed by an anti-human horseradish peroxidase-conjugated antibody. anti-human horseradish peroxidase-conjugated antibody .suggested: NoneThe cells were formalin-fixed and ethanol permeabilized followed by incubation with a murine monoclonal antibody which targets the viral nucleocapsid protein (Sino Biological), followed by a secondary anti-mouse IgG peroxidase conjugate (Thermo Scientific) and TrueBlue (KPL) substrate. anti-mouse IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Cells and viruses: VeroE6 cells (ATCC, CRL-1586) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (v/v VeroE6suggested: NoneSoftware and Algorithms Sentences Resources Images of all wells were acquired by a CTL Immunospot analyzer, equipped with Biospot® software to quantitate the nucleocapsid-positive cells (= virus signal). Biospot®suggested: NoneVirus titer and viral RNA from animals were analyzed using GraphPad Prism v 9.1.0. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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