ALG-097111, a potent and selective SARS-CoV-2 3-chymotrypsin-like cysteine protease inhibitor exhibits in vivo efficacy in a Syrian Hamster model

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1. Version published to 10.1016/j.bbrc.2021.03.096

   May 1, 2021
2. 

   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2021.02.14.431129: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Human β-coronavirus OC43 assay: The human beta-coronavirus OC43 assay in HeLa cells was performed as previously described.	HeLa suggested: CLS Cat# 300194/p772_HeLa, RRID:CVCL_0030)
   [13] Human α-Coronavirus 229E assay: The human alpha-coronavirus 229E was purchased from Virapur (San Diego, CA) and propagated using MRC-5 human lung fibroblast cells (ATCC).	MRC-5 suggested: ICLC Cat# HL95001, RRID:CVCL_0440)
   Huh7 cells (JCRB cell Bank) were …

   SciScore for 10.1101/2021.02.14.431129: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Human β-coronavirus OC43 assay: The human beta-coronavirus OC43 assay in HeLa cells was performed as previously described.	HeLa suggested: CLS Cat# 300194/p772_HeLa, RRID:CVCL_0030)
   [13] Human α-Coronavirus 229E assay: The human alpha-coronavirus 229E was purchased from Virapur (San Diego, CA) and propagated using MRC-5 human lung fibroblast cells (ATCC).	MRC-5 suggested: ICLC Cat# HL95001, RRID:CVCL_0440)
   Huh7 cells (JCRB cell Bank) were cultured using DMEM media, supplemented with 10 % fetal bovine serum (FBS), 1% (v/v	Huh7 suggested: None
   SARS-CoV-2 nanoluciferase assay in human ACE-2 expressing A549 cells: The SARS-CoV-2 nanoluciferase assay using A549 cells expressing the human ACE-2 receptor was performed in the laboratory of Pei-Yong Shi at the University of Texas, Medical Branch [14] on behalf of Aligos Therapeutics, Inc.	A549 suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   SARS-CoV-2 3CLpro and human cathepsin L biochemical assays: The SARS-CoV-2 3CLpro and human cathepsin L assays were performed as previously described [12].	SARS-CoV-2 3CLpro suggested: None

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • No funding statement was detected.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2021.02.14.431129v1 on bioRxiv

   Feb 15, 2021