Stroke increases the expression of ACE2, the SARS-CoV-2 binding receptor, in murine lungs
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SciScore for 10.1101/2020.06.24.162941: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable The C57BL/6Hsd wild type male mice are received from Envigo, Netherlands. Table 2: Resources
Antibodies Sentences Resources Primary antibodies used were goat anti-mouse ACE2 (AF3437, R and D Systems), goat anti-mouse ACE (AF1513, R&D Systems), and rabbit anti-mouse actin (4967, Cell Signaling). anti-mouse ACE2suggested: Noneanti-mouse ACEsuggested: (R and D Systems Cat# AF1513, RRID:AB_354832)anti-mouse actin ( 4967 , Cell Signaling) .suggested: NoneAfter extensive washing (three times for 15 min each in TBS containing 0.1% Tween 20), proteins were … SciScore for 10.1101/2020.06.24.162941: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable The C57BL/6Hsd wild type male mice are received from Envigo, Netherlands. Table 2: Resources
Antibodies Sentences Resources Primary antibodies used were goat anti-mouse ACE2 (AF3437, R and D Systems), goat anti-mouse ACE (AF1513, R&D Systems), and rabbit anti-mouse actin (4967, Cell Signaling). anti-mouse ACE2suggested: Noneanti-mouse ACEsuggested: (R and D Systems Cat# AF1513, RRID:AB_354832)anti-mouse actin ( 4967 , Cell Signaling) .suggested: NoneAfter extensive washing (three times for 15 min each in TBS containing 0.1% Tween 20), proteins were detected with horseradish peroxidase-coupled anti-goat or anti-rabbit antibody. anti-goatsuggested: Noneanti-rabbitsuggested: NoneExperimental Models: Organisms/Strains Sentences Resources The C57BL/6Hsd wild type male mice are received from Envigo, Netherlands. C57BL/6Hsdsuggested: RRID:MGI:2161086)Software and Algorithms Sentences Resources Densitometry analysis was performed using ImageJ software (NIH, USA), and a β-actin control was used to confirm equal sample loading and normalization of the data. ImageJsuggested: (ImageJ, RRID:SCR_003070)Stained samples were measured on BD FACSAria™ flow cytometer and analyzed using FlowJo software (BD Biosciences). FlowJosuggested: (FlowJo, RRID:SCR_008520)2.6 Statistical analysis: Data were analyzed using GraphPad Prism version 6.0. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT04335136 Completed Recombinant Human Angiotensin-converting Enzyme 2 (rhACE2) a… Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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