Brequinar and dipyridamole in combination exhibits synergistic antiviral activity against SARS-CoV-2 in vitro: Rationale for a host-acting antiviral treatment strategy for COVID-19

Article activity feed

1. Version published to 10.1016/j.antiviral.2022.105403

   Oct 1, 2022
2. 

   ScreenIT

   Apr 3, 2022

   SciScore for 10.1101/2022.03.30.486499: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	Contamination: The absence of mycoplasma contamination was validated regularly with a PCR-based method (ATCC, Universal Mycoplasma Detection Kit, 30-1012K).

   Table 2: Resources

   Antibodies
   Sentences	Resources
   To detect infection, plates were incubated overnight at 4 ⁰C with a rabbit anti-N protein monoclonal antibody (SioBiological 40143-R004) diluted 1:20,000.	anti-N protein suggested: None
   Plates were washed and treated with an AF488-conjugated goat anti-rabbit secondary antibody for 2 h at room temperature.	anti-rabbit suggested: None
   …

   SciScore for 10.1101/2022.03.30.486499: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	Contamination: The absence of mycoplasma contamination was validated regularly with a PCR-based method (ATCC, Universal Mycoplasma Detection Kit, 30-1012K).

   Table 2: Resources

   Antibodies
   Sentences	Resources
   To detect infection, plates were incubated overnight at 4 ⁰C with a rabbit anti-N protein monoclonal antibody (SioBiological 40143-R004) diluted 1:20,000.	anti-N protein suggested: None
   Plates were washed and treated with an AF488-conjugated goat anti-rabbit secondary antibody for 2 h at room temperature.	anti-rabbit suggested: None
   Software and Algorithms
   Sentences	Resources
   Infection efficiency, defined as GFP-positive cells divided by total nuclei, was calculated for each image using a CellProfiler pipeline.	CellProfiler suggested: None

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
   

   Identifier	Status	Title
   NCT05166876	Recruiting	Brequinar Combined With Dipyridamole in Patients With Mild t…

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • No conflict of interest statement was detected. If there are no conflicts, we encourage authors to explicit state so.
   • No funding statement was detected.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Read the original source
3. 

   Version published to 10.1101/2022.03.30.486499 on bioRxiv

   Mar 31, 2022