Unraveling the antiviral activity of plitidepsin against SARS-CoV-2 by subcellular and morphological analysis

  1. Martin Sachse
  2. Raquel Tenorio
  3. Isabel Fernández de Castro
  4. Jordana Muñoz-Basagoiti
  5. Daniel Perez-Zsolt
  6. Dàlia Raïch-Regué
  7. Jordi Rodon
  8. Alejandro Losada
  9. Pablo Avilés
  10. Carmen Cuevas
  11. Roger Paredes
  12. Joaquim Segalés
  13. Bonaventura Clotet
  14. Júlia Vergara-Alert
  15. Nuria Izquierdo-Useros
  16. Cristina Risco

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Abstract

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  1. Version published to 10.1016/j.antiviral.2022.105270
  2. ScreenIT

    SciScore for 10.1101/2021.12.16.472880: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    N protein was labeled with the rabbit anti-SARS-CoV-2 nucleocapsid antibody (GXT 135357 GeneTex) diluted 1/50 in TBG for 1h.
    anti-SARS-CoV-2
    suggested: None
    Double stranded RNA (dsRNA), an intermediate of viral replication, was labeled with the mouse monoclonal anti-dsRNA J2 antibody (10010200, English and Scientific Consulting Kft, SCICONS) diluted 1/30 in TBG for 1h.
    anti-dsRNA J2
    suggested: (SCICONS Cat# 10010200, RRID:AB_2651015)
    Immunogold cytochemical control of anti-nucleocapsid (N) antibody in non-infected Vero E6 cells, with and without plitidepsin.
    anti-nucleocapsid (N
    suggested: None
    Ultrathin sections of cells were incubated with anti-N primary antibody followed by a secondary antibody conjugated with 10 nm colloidal gold particles and visualized by TEM.
    anti-N
    suggested: None
    Immunogold cytochemical control of anti-dsRNA antibody in non-infected Vero E6 cells, with and without plitidepsin.
    anti-dsRNA
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    The virus was propagated for two passages and a virus stock was prepared collecting the supernatant from Vero E6 cell and sequenced as described elsewhere (Rodon et al., 2021).
    Vero E6
    suggested: None
    Software and Algorithms
    SentencesResources
    Biosafety Approval: The biologic biosafety committee of the Research Institute Germans Trias i Pujol approved the execution of SARS-CoV-2 experiments at the BSL3 laboratory of the Center for Bioimaging and Comparative Medicine, CMCiB (CSB-20-015-M3)
    Bioimaging
    suggested: None

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: We found the following clinical trial numbers in your paper:

    IdentifierStatusTitle
    NCT04784559RecruitingTrial to Determine the Efficacy/Safety of Plitidepsin vs Con…

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.12.16.472880 on bioRxiv