Ionophore antibiotic X-206 is a potent inhibitor of SARS-CoV-2 infection in vitro

  1. Esben B. Svenningsen
  2. Jacob Thyrsted
  3. Julia Blay-Cadanet
  4. Han Liu
  5. Shaoquan Lin
  6. Jaime Moyano-Villameriel
  7. David Olagnier
  8. Manja Idorn
  9. Søren R. Paludan
  10. Christian K. Holm
  11. Thomas B. Poulsen

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Abstract

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  1. Version published to 10.1016/j.antiviral.2020.104988
  2. Version published to 10.1101/2020.06.14.149153v2 on bioRxiv
  3. ScreenIT

    SciScore for 10.1101/2020.06.14.149153: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line AuthenticationContamination: All cell lines were regularly tested for mycoplasma contamination by sequencing from GATC Biotech (Germany).

    Table 2: Resources

    Antibodies
    SentencesResources
    (Gene Tex) and Rabbit anti-Vinculin #13901 (E1E9V XP®) (
    anti-Vinculin
    suggested: (Cell Signaling Technology Cat# 13901, RRID:AB_2728768)
    Experimental Models: Cell Lines
    SentencesResources
    Viral replication Assay: Vero E6 cells expressing hTMPRSS2 were a kind gift of Makoto Takeda (University of Tokyo, Japan), and were cultured in DMEM (Lonza) supplemented with 10% heat inactivated fetal calf serum, 200 IU/mL penicillin, 100 μg/mL streptomycin, 600 μg/mL glutamine and 10 μg/mL blasticidin.
    Vero E6
    suggested: RRID:CVCL_XD71)
    The virus was propagated in Vero-hTMPRSS2 cells.
    Vero-hTMPRSS2
    suggested: None
    3 Western blot analysis: Vero E6 hTMPRSS2 cells infected with SARS-Cov-2 were lysed and analyzed by Western blot as done in with Rabbit Anti-SARS CoV-2 (COVID-19) Spike (Cat No. GTX135356)
    Vero E6 hTMPRSS2
    suggested: None
    Software and Algorithms
    SentencesResources
    Cells with condensed chromatin were counted in CellProfiler 3.1.9.
    CellProfiler
    suggested: None
    The count for the 8 sites each well was summed, all wells were subtracted the mean count from uninfected wells (10 wells), normalized to DMSO/media treated cells and fitted to a 4-parameter nonlinear regression in Prism 8.3.0 for Windows, GraphPad Software, La Jolla California USA,
    Prism
    suggested: (PRISM, RRID:SCR_005375)
    Data was subtracted background (CellTiter blue in media, no cells) normalized to DMSO/media treated cells and fitted to a 4-parameter nonlinear regression in Prism 8.3.0 for Windows, GraphPad Software, La Jolla California USA,
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  4. Version published to 10.1101/2020.06.14.149153v1 on bioRxiv