Immunoglobulin fragment F(ab’)2 against RBD potently neutralizes SARS-CoV-2 in vitro

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1. Version published to 10.1016/j.antiviral.2020.104868

   Oct 1, 2020
2. 

   ScreenIT

   Jun 5, 2020

   SciScore for 10.1101/2020.04.07.029884: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: 3.14 Ethics statements: All animal experiments were performed strictly according to the Regulations for the Administration of Affairs Concerning Experimental Animals in China, and the protocols were approved by the Laboratory Animal Care and Use Committee of Wuhan Institute of Virology, Chinese Academy of Sciences (Wuhan, China).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	3.6 Mouse immunization and sampling: Female BALB/c mice aged 6-8 weeks were housed in specific pathogen-free animal care facilities.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   After that, …

   SciScore for 10.1101/2020.04.07.029884: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: 3.14 Ethics statements: All animal experiments were performed strictly according to the Regulations for the Administration of Affairs Concerning Experimental Animals in China, and the protocols were approved by the Laboratory Animal Care and Use Committee of Wuhan Institute of Virology, Chinese Academy of Sciences (Wuhan, China).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	3.6 Mouse immunization and sampling: Female BALB/c mice aged 6-8 weeks were housed in specific pathogen-free animal care facilities.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   After that, cells were blocked with 2% nonfat-milk at room temperature for 1 hour, then were washed with PBS and incubated with rabbit anti-NP antibodies at room temperature for 2 hours.	anti-NP suggested: None
   Cells were washed again before incubation with goat anti-rabbit Alexa Fluor 488-conjugated antibodies at room temperature for 1 hour.	anti-rabbit suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   3.1 Cells lines and viruses: Vero E6 and HeLa cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS, Gibco) at 37°C with 5% CO2.	Vero E6 suggested: None
   PcDNA3.1-human ACE2 was transfected into HeLa cells with Lipo2000 (Invitrogen), PcDNA3.1 was made as control.	HeLa suggested: None
   Vero-E6 cells were seeded in 48-well plates with 5 × 104 cells/well overnight.	Vero-E6 suggested: None
   3.13 Purification of RBD-Specific F(ab’)2: For purification of RBD-specific F(ab’)2, RBD protein expressed by CHO cells and prepared as described above, was coupled on pre-activated resin (PabPurSulfolink Beads, SMART Life Sciences, Changzhou) through amino reaction17.	CHO suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   3.6 Mouse immunization and sampling: Female BALB/c mice aged 6-8 weeks were housed in specific pathogen-free animal care facilities.	BALB/c suggested: None
   Software and Algorithms
   Sentences	Resources
   3.13 Purification of RBD-Specific F(ab’)2: For purification of RBD-specific F(ab’)2, RBD protein expressed by CHO cells and prepared as described above, was coupled on pre-activated resin (PabPurSulfolink Beads, SMART Life Sciences, Changzhou) through amino reaction17.	SMART suggested: (SMART, RRID:SCR_005026)
   3.15 Data analysis: Data was analyzed using GraphPad Prism 8.0 software (San Diego, CA, USA), are presented as mean±SD based on at least three independent experiments.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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3. 

   Version published to 10.1101/2020.04.07.029884v1 on bioRxiv

   Apr 9, 2020