Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters

Version published to 10.1016/j.ajpath.2021.10.009

Feb 1, 2022

SciScore for 10.1101/2021.06.25.449918: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Ethics	Euthanasia Agents: At study endpoints, animals were euthanized with intraperitoneal sodium pentobarbital. IACUC: The animal procedures in this study were in accordance with the principles set forth by the Institutional Animal Care and Use Committee at Johns Hopkins University and the National Research Council’s Guide for the Care and Use of Laboratory Animals (8th edition) SARS-CoV-2
Sex as a biological variable	Animals: 7 to 8 week old male and female golden Syrian hamsters Mesocricetus auratus (Envigo) were singly housed in the JHU CRB Animal Biosafety Level 3 (ABSL)-3 facility.
Randomization	not detected.
Blinding	Histopathological analysis was performed independently by two veterinary pathologists (S.B. and K.M.) blinded to animal identification and infection status.
Power Analysis	not detected.
Cell Line Authentication	not detected.

Table 2: Resources

Antibodies
Sentences	Resources
Immunohistochemistry: 10% NBF-fixed lung sections of SARS-CoV-2 infected and control animals were immunostained with anti-Iba-1 antibody (1:2000; Wako; 019-19741 Richmond, VA)	anti-Iba-1 suggested: (FUJIFILM Wako Shibayagi Cat# 016-20001, RRID:AB_839506)
anti-CD3 antibody (1:200; DAKO; Ref: A0452 Santa Clara, CA, or anti-pan-cytokeratin antibody (1:1000; Santa Cruz; sc-8018 Dallas, TX)	anti-CD3 suggested: (Agilent Cat# A0452, RRID:AB_2335677) anti-pan-cytokeratin suggested: (Covance Cat# SIG-3464-1000, RRID:AB_663022)
Slides were then stained with anti-Iba1 antibody (1:2000; Wako; 019- 19741 Richmond, VA) using the Bond Polymer Refine Red Kit (cat.	anti-Iba1 suggested: (FUJIFILM Wako Shibayagi Cat# 019-19741, RRID:AB_839504)
Experimental Models: Cell Lines
Sentences	Resources
Briefly, tissue homogenates were serially diluted 10-fold, transferred in sextuplicate into 96-well plates confluent with Vero-E6-TMPRSS2 cells (obtained from the Japan Institute of Infectious Diseases), incubated at 370C for 4 days, and then stained with naphthol blue-black solution for visualization.	Vero-E6-TMPRSS2 suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
Software and Algorithms
Sentences	Resources
Data were analyzed using FlowJo 10.0.8 software (FlowJo, LLC, Ashland, OR)	FlowJo suggested: (FlowJo, RRID:SCR_008520)
Statistical analysis: Statistical analyses were performed using GraphPad Prism version 7.04.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

Results from TrialIdentifier: No clinical trial numbers were referenced.

Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

Results from JetFighter: We did not find any issues relating to colormaps.

Results from rtransparent:

Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
No funding statement was detected.
No protocol registration statement was detected.

Results from scite Reference Check: We found no unreliable references.

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Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters

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