Virucidal activity and mechanism of action of cetylpyridinium chloride against SARS-CoV-2

Article activity feed

1. Version published to 10.1016/j.ajoms.2022.04.001

   Nov 1, 2022
2. 

   ScreenIT

   Feb 2, 2022

   SciScore for 10.1101/2022.01.27.477964: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Next, membranes were washed 3 times in PBS-T, incubated with horseradish peroxidase-conjugated anti-rabbit antibody (Abcam, Cambridge, UK) at 1:17,000 dilution for 1 h, washed again 3 times in PBS-T, incubated with SuperSignal West Dura reagent (Thermo Fisher Scientific), and imaged using an Amersham Imager 680 (Cytiva, Tokyo, Japan). 2.4 Interaction of the spike protein and ACE2: The interaction between the receptor-binding domain (RBD) of the SARS-CoV-2 S protein and ACE2 was …

   SciScore for 10.1101/2022.01.27.477964: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Next, membranes were washed 3 times in PBS-T, incubated with horseradish peroxidase-conjugated anti-rabbit antibody (Abcam, Cambridge, UK) at 1:17,000 dilution for 1 h, washed again 3 times in PBS-T, incubated with SuperSignal West Dura reagent (Thermo Fisher Scientific), and imaged using an Amersham Imager 680 (Cytiva, Tokyo, Japan). 2.4 Interaction of the spike protein and ACE2: The interaction between the receptor-binding domain (RBD) of the SARS-CoV-2 S protein and ACE2 was evaluated using a COVID-19 Spike-ACE2 Binding Assay Kit II (RayBiotech Life, Peachtree Corners, GA, USA).	anti-rabbit suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   The virus was propagated in VeroE6 cells expressing transmembrane protease serine 2 (TMPRSS2).	VeroE6 suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
   After dilution, the virus solutions were serially diluted in 10-fold steps using serum-free DMEM and then inoculated onto VeroE6/TMPRSS2 cell monolayers in a 12-well plate.	VeroE6/TMPRSS2 suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
   Software and Algorithms
   Sentences	Resources
   IBM SPSS Statistics 27 was used for statistical analysis.	SPSS suggested: (SPSS, RRID:SCR_002865)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • No funding statement was detected.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Read the original source
3. 

   Version published to 10.1101/2022.01.27.477964 on bioRxiv

   Jan 31, 2022