A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS-CoV-2 Spike S1 RBD produced in Escherichia coli

  1. Marcelo S. Conzentino
  2. Ana C. A. Gonçalves
  3. Nigella M. Paula
  4. Fabiane G. M. Rego
  5. Dalila L. Zanette
  6. Mateus N. Aoki
  7. Jeanine M. Nardin
  8. Luciano Fernandes Huergo

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Abstract

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  1. Version published to 10.1007/s42770-022-00753-x
  2. ScreenIT

    SciScore for 10.1101/2021.12.22.21268286: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsIRB: The Institutional Ethics Review Board CEP/HEG (n# 31592620.4.1001.0098 and n# 54095221.0.0000.0098), CEP/UFPR (n# 43948621.7.0000.0102 and n# 35872520.8.0000.0102) approved this study.
    Consent: Informed consent was obtained from all participants in this study.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.

    Table 2: Resources

    Recombinant DNA
    SentencesResources
    Expression and purification of recombinant receptor-binding domain of SARS-CoV-2 spike protein: A codon optimized synthetic gene was produced and cloned into the pET28a vector by General Biosystems.
    pET28a
    suggested: RRID:Addgene_114145)
    The plasmid construction was named pLHSarsCoV2-S and allowed the expression of a N-terminal 6xHis tagged fusion of a fragment of SARS-CoV-2 Spike S1 containing part of the N-terminal domain and the full RBD (Uniprot MN908947, M153 to T589).
    pLHSarsCoV2-S
    suggested: None

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.12.22.21268286v1 on medRxiv