The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel
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SciScore for 10.1101/2020.06.18.20134304: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The in-house indirect ELISA was validated using a panel of SARS-CoV-2-positive sera previously tested by commercial anti-SARS-CoV-2 IgG lateral flow immunochromatography (various commercial brands). 2.6. Ethical Considerations: This work was approved by the UFMG’s Ethics Committee and by the National Research Ethics’ Committee, under number CAAE 31686320.0.000.5149. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Additionally, serum samples from all laboratory members were also collected approximately 45 days after the beginning of the study, to … SciScore for 10.1101/2020.06.18.20134304: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The in-house indirect ELISA was validated using a panel of SARS-CoV-2-positive sera previously tested by commercial anti-SARS-CoV-2 IgG lateral flow immunochromatography (various commercial brands). 2.6. Ethical Considerations: This work was approved by the UFMG’s Ethics Committee and by the National Research Ethics’ Committee, under number CAAE 31686320.0.000.5149. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Additionally, serum samples from all laboratory members were also collected approximately 45 days after the beginning of the study, to assess the presence of antibodies against SARS-CoV-2. SARS-CoV-2suggested: NoneSoftware and Algorithms Sentences Resources Results obtained were analyzed in the QuantStudio™ Design and Analysis software (v.1.5.1) and graphs were generated using the GraphPad Prism software (v.8.4.2). 2.4. Detection of Viral RNA from samples stored in the Viral Transport Medium and the Denaturing Solution: To evaluate the preservation of the viral RNA in VTM versus DS, clinical samples were collected from hospitalized COVID-19 suspected patients using either DS or VTM. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Our study have important limitations. First, this was not a case-control study, as our results were not compared to those obtained in a diagnostic laboratory routinely receiving clinical samples in VTM. Nonetheless, the differences in the possible extent of live virus exposition when VTM or DS are used are obvious. In this regard, the fact that none of our laboratory members was either infected or even seroconverted is an important indication that DS has been helpful in avoiding nosocomial exposition. Another limitation is that we are not able to quantify to which extent the good laboratory practices adopted in the laboratory could also be responsible for the verified results. Nonetheless, the use of the denaturing transport media is a critical part of such practices. Finally, we attempted to quantify the extent of SARS-CoV-2 inactivation using the described DS. To that end, isolated, laboratory cultivated live viruses were loaded on VTM or DS and plated on VERO cells. Viruses loaded on VTM remained replicative and able to generate plaques in the cellular monolayers (not shown). On the other hand, even when highly diluted, the guanidine salt present in DS was extremely toxic to cells, and the monolayers were destroyed before any eventual plaque could form. Nevertheless, it seems obvious to assume that, like cells, viruses were equally inactivated during exposition to DS. In spite of the limitations of our study, the use of denaturing, virus-inactivation solution as a collecti...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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