Temporal landscape of mutational frequencies in SARS-CoV-2 genomes of Bangladesh: possible implications from the ongoing outbreak in Bangladesh

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Abstract

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  1. SciScore for 10.1101/2020.08.20.259721: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    NIH rigor criteria are not applicable to paper type.

    Table 2: Resources

    Software and Algorithms
    SentencesResources
    Later, the MSA file was opened with Jalview visualization software to eliminate the redundancy of the studied sequences (Waterhouse et al., 2009).
    Jalview
    suggested: (Jalview, RRID:SCR_006459)
    2.3 Phylogenetic Analysis of SARS-CoV-2 sequences: To infer the evolutionary relationships among the examined sequences, the sequences were aligned with relevant reference sequences retrieved from NCBI database using the neighbor-joining approach (Rahman et al., 2020).
    NCBI
    suggested: (NCBI, RRID:SCR_006472)
    The Molecular Evolutionary Genetics Analysis across Computing Platforms (MEGA 7) (Kumar et al., 2016) software was used to construct phylogenetic tree applying the neighbor-joining method (Saha et al., 2020) and evolutionary distances were computed using the Kimura-Nei method (Saitou, 1987).
    MEGA
    suggested: (Mega BLAST, RRID:SCR_011920)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).


    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.


    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.


    Results from JetFighter: We did not find any issues relating to colormaps.


    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.