Safety and Pharmacokinetics of Intranasally Administered Heparin
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SciScore for 10.1101/2021.07.05.21259936: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IACUC: Animals: All procedures were approved by the University of Mississippi Institutional Animal Care and Use Committee (protocol #19-009) or the University of Mississippi Medical Center Institutional Animal Care and Use Committee (protocol #1571).
Field Sample Permit: All animals for toxicology studies were housed in the AALAC-accredited University of Mississippi Animal Facility and given access to rodent chow (Cat.# 7001, Envigo Teklad 4% Fat Rodent Diet) and tap water ad libitum for the duration of the experiments.
Euthanasia Agents: Upon completion of the study, mice were anesthetized with vaporized isofluorane, perfused with phosphate buffer saline, and secondarily euthanized with …SciScore for 10.1101/2021.07.05.21259936: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IACUC: Animals: All procedures were approved by the University of Mississippi Institutional Animal Care and Use Committee (protocol #19-009) or the University of Mississippi Medical Center Institutional Animal Care and Use Committee (protocol #1571).
Field Sample Permit: All animals for toxicology studies were housed in the AALAC-accredited University of Mississippi Animal Facility and given access to rodent chow (Cat.# 7001, Envigo Teklad 4% Fat Rodent Diet) and tap water ad libitum for the duration of the experiments.
Euthanasia Agents: Upon completion of the study, mice were anesthetized with vaporized isofluorane, perfused with phosphate buffer saline, and secondarily euthanized with decapitation.
IRB: Human Study Population: An Early Phase 1 interventional clinical trial (NCT04490239) in a small sample population was performed under the approval and guidance of the University of Mississippi Institutional Review Board (protocol #21-004).
Consent: All subjects submitted written informed consent prior to enrollment in the study.Sex as a biological variable Toxicology experiments were performed on wild-type male (19-23g) and female (16-19g) C57BL6 mice from Envigo (Indianapolis, IN) in study groups of six mice for each dose (three male, three female). Randomization On test day, the subject was placed in a test chamber containing 3 cm of fresh 1/8 in corncob bedding for a 15-minute trial, with the food stimulus buried approximately 1 cm below the bedding surface in a randomly assigned corner of the cage. Blinding Platelets in each image were counted by a blinded observer. Power Analysis The study was powered to preserve an 80% chance of not committing a Type II error with a 5% chance of Type I error at significant effect sizes (weight as the variable: power > 0.8 to detect a 20% change in body weight within sex (n = 3), 10% change in body weight within entire group (n = 6); aPTT time: power > 0.8 to detect a 100% change in aPTT (n = 4); buried food recovery time: power > 0.8 to detect a 100% change in recovery time (n = 5)). Cell Line Authentication Authentication: Signs of inflammation, bleeding, and/or nasal discharge were monitored twice daily by visual inspection of the nose, body, cage, and bedding. Table 2: Resources
Experimental Models: Organisms/Strains Sentences Resources Toxicology experiments were performed on wild-type male (19-23g) and female (16-19g) C57BL6 mice from Envigo (Indianapolis, IN) in study groups of six mice for each dose (three male, three female). C57BL6suggested: NoneIn vivo imaging experiments were performed on three female SKH1-Elite mice (Crl:SKH1-Hrhr Outbred: Charles River Laboratories, Wilmington, MA). SKH1-Elitesuggested: NoneSoftware and Algorithms Sentences Resources One-phase and two-phase exponential decay modeling was carried out using GraphPad Prism 8.4.3 and hypothesis testing using an extra sum-of-squares F test was performed. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Data were analyzed using SPSS and SyStat SigmaPlot v13 software. SPSSsuggested: (SPSS, RRID:SCR_002865)SyStatsuggested: (SyStat, RRID:SCR_010455)SigmaPlotsuggested: (SigmaPlot, RRID:SCR_003210)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT04490239 Completed Intranasal Heparin Tolerability Study Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 18. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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