Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemiluminescent immunoassays

  1. Arantxa Valdivia
  2. Ignacio Torres
  3. Víctor Latorre
  4. Clara Francés-Gómez
  5. Eliseo Albert
  6. Roberto Gozalbo-Rovira
  7. María Jesús Alcaraz
  8. Javier Buesa
  9. Jesús Rodríguez-Díaz
  10. Ron Geller
  11. David Navarro

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Abstract

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  1. Version published to 10.1007/s10096-020-04128-8
  2. ScreenIT

    SciScore for 10.1101/2020.09.07.20188151: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: This study was approved by the Research Ethics Committee of Hospital Clínico Universitario INCLIVA (March 2020). 3.2.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    SARS-CoV-2 Neutralizing antibody assay: A Green fluorescent protein (GFP) reporter-based pseudotyped virus neutralization assay using a non-replicative vesicular stomatitis virus (VSV lacking the G protein) backbone coated with the SARS-CoV-2 spike (S) protein was used for neutralization assays on Vero cells, as previously described [24].
    GFP
    suggested: None
    The LIAISON SARS-CoV-2 S1/S2 IgG chemiluminescent assay (DiaSorin S.p.A., Saluggia, Italy) detects IgG antibodies directed against a recombinant S protein (S1/S2).
    DiaSorin S.p.A., Saluggia, Italy) detects IgG
    suggested: None
    The MAGLUMI 2019-nCoV IgG is an indirect CLIA for assessment of IgG antibodies against SARS-CoV-2 S and nucleocapsid (N) proteins on the fully automated MAGLUMI analyzers (SNIBE – Shenzhen New Industries Biomedical Engineering Co., Ltd, Shenzhen, China).
    SARS-CoV-2
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    SARS-CoV-2 Neutralizing antibody assay: A Green fluorescent protein (GFP) reporter-based pseudotyped virus neutralization assay using a non-replicative vesicular stomatitis virus (VSV lacking the G protein) backbone coated with the SARS-CoV-2 spike (S) protein was used for neutralization assays on Vero cells, as previously described [24].
    Vero
    suggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)
    Software and Algorithms
    SentencesResources
    The analyses were performed using SPSS version 20.0 (SPSS, Chicago, IL, USA).
    SPSS
    suggested: (SPSS, RRID:SCR_002865)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.09.07.20188151v1 on medRxiv