Evaluating the serological status of COVID-19 patients using an indirect immunofluorescent assay, France

  1. S. Edouard
  2. P. Colson
  3. C. Melenotte
  4. F. Di Pinto
  5. L. Thomas
  6. B. La Scola
  7. M. Million
  8. H. Tissot-Dupont
  9. P. Gautret
  10. A. Stein
  11. P. Brouqui
  12. P. Parola
  13. J.-C. Lagier
  14. D. Raoult
  15. Michel Drancourt

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Abstract

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  1. Version published to 10.1007/s10096-020-04104-2
  2. ScreenIT

    SciScore for 10.1101/2020.05.05.20092064: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: The non-interventional nature of this study was approved by the Ethical Committee of the IHU Méditerranée Infection under no. 2020-13.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Indirect immunofluorescence assay: Anti-SARS-Cov 2 antibodies were detected using an in house indirect immunofluorescence assay (IFA), as previously described (9).
    Anti-SARS-Cov 2
    suggested: (GenWay Biotech Inc. Cat# GWB-2A8325, RRID:AB_10308561)
    In a first step, each serum sample was screened for the presence of anti-SARS CoV-2 antibodies using the IFA, as previously described (9).
    anti-SARS CoV-2
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Vero E6 cells (ATCC CRL-1586, Rockville, MD, USA) infected with the SARS-CoV2 strain IHU-MI2 (full genome sequence of this strain was deposited under the European Molecular Biology Laboratory EMBL project accession no. PRJEB38023) (10) were harvested between 24 hours and 48 hours post-inoculation when cytopathic effect begins to be observed before massive cell lyses begin, washed with sterile phosphate buffered saline (PBS) (Oxoid, Dardilly, France) and inactivated using 5% paraformaldehyde.
    Vero E6
    suggested: None
    Fifty nanolitres of uninfected Vero cells were also spotted on each well as a negative control and a clinical isolate of Staphylococcus aureus (identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry) (11) was spotted on each well in order to ensure further serum deposition, as previously described (12).
    Vero
    suggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)
    Software and Algorithms
    SentencesResources
    For the data comparisons and statistical analyses, Fisher’s exact test or the Chi-squared test and standard statistical software (GraphPad Prism 5) were used.
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)
    ROC curves were calculated using GraphPad Prism 5.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.05.05.20092064 on medRxiv