Spike vs nucleocapsid SARS-CoV-2 antigen detection: application in nasopharyngeal swab specimens
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SciScore for 10.1101/2021.03.08.21253148: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The study was approved by the SMC institutional review board committee (approval number – 7769-20-SMC). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antigens and antibodies: Nucleocapsid – SARS-CoV-2 nucleocapsid phosphoprotein’s sequence (GenBank accession: YP_009724397.2) was codon-optimized for E. coli expression, synthesized and cloned (EcoRI, NotI) in pET-28a(+) expression vector, by twist bioscience (twist bioscience, USA). Antigenssuggested: NoneAntibodies: Polyclonal anti NC protein antibodies were … SciScore for 10.1101/2021.03.08.21253148: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The study was approved by the SMC institutional review board committee (approval number – 7769-20-SMC). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antigens and antibodies: Nucleocapsid – SARS-CoV-2 nucleocapsid phosphoprotein’s sequence (GenBank accession: YP_009724397.2) was codon-optimized for E. coli expression, synthesized and cloned (EcoRI, NotI) in pET-28a(+) expression vector, by twist bioscience (twist bioscience, USA). Antigenssuggested: NoneAntibodies: Polyclonal anti NC protein antibodies were prepared by immunizing rabbits with 100μg of purified protein in incomplete freund’s adjuvant by subcutaneous injection. anti NC proteinsuggested: NoneMonoclonal anti SARS-CoV-2 RBD, NTD and NC antibodies were isolated from a phage-display library, constructed from COVID-19 patients showing substantial illness and panned against plate adhered spike and nucleocapsid as previously described (18, 19, 22). Monoclonalsuggested: NoneNCsuggested: NoneAntibody labeling: Biotinylation of IgG purified antibody fractions was carried out using sulfo-NHS-SS-biotin [sulfosuccinimidyl-2-(biotinamido) ethyl-1,3-dithiopropionate; Pierce 21331] according to the manufacturer’s instructions. sulfo-NHS-SS-biotin [sulfosuccinimidyl-2-suggested: NoneA calculated average number of 4 biotin molecules per antibody was determined by the HABA ([2-(4-hydroxyazobenzene] benzoic acid) method (Pierce 28050). 4-hydroxyazobenzene] benzoic acid) method (Pierce 28050suggested: NoneExperimental Models: Cell Lines Sentences Resources SARS-CoV-2 virus was cultivated as described in (21) on VERO E6 cells. VERO E6suggested: RRID:CVCL_XD71)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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